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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-9-1
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pubmed:abstractText |
A Cycling Probe Technology (CPT) assay with a lateral-flow device (strip) was developed for the detection of the mecA gene from methicillin-resistant Staphylococcus aureus (MRSA) cultures. The assay uses a mecA probe (DNA-RNA-DNA) labeled with fluorescein at the 5' terminus and biotin at the 3' terminus. The CPT reaction occurs at a constant temperature, which allows the probe to anneal to the target DNA. RNase H cuts the RNA portion of the probe, allowing the cleaved fragments to dissociate from the target DNA, making the target available for further cycling. The strip detection step uses a nitrocellulose membrane with streptavidin and immunoglobulin G antibody impregnated on the surface. In the absence of the mecA gene, the uncut probe is bound to an antifluorescein-gold conjugate and is then captured by the streptavidin to form a test line. In the presence of the mecA gene, the probe is cut and no test line is formed on the strip. A screen of 324 S. aureus clinical isolates by the CPT-strip assay showed a 99.4% sensitivity and a 100% specificity compared to the results of PCR for the detection of the mecA gene. Specificity testing showed that the CPT-strip assay did not exhibit any cross-reactivity with a panel of mecA-negative non-S. aureus isolates. The CPT-strip assay is simple and does not require sophisticated equipment. Furthermore, the assay takes 1.5 h starting from a primary culture to the time to detection of the mecA gene in S. aureus isolates.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-10354856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-10369744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-10369748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-1629327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-1939577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-2400595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-7559973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-8452155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-8647774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-8712771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-8940435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9025087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9158769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9182101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9336672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9665984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10878037-9705407
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2525-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10878037-Bacterial Proteins,
pubmed-meshheading:10878037-Bacteriological Techniques,
pubmed-meshheading:10878037-Culture Media,
pubmed-meshheading:10878037-DNA Probes,
pubmed-meshheading:10878037-Humans,
pubmed-meshheading:10878037-Immunoassay,
pubmed-meshheading:10878037-Methicillin Resistance,
pubmed-meshheading:10878037-Polymerase Chain Reaction,
pubmed-meshheading:10878037-RNA Probes,
pubmed-meshheading:10878037-Reagent Strips,
pubmed-meshheading:10878037-Sensitivity and Specificity,
pubmed-meshheading:10878037-Staphylococcal Infections,
pubmed-meshheading:10878037-Staphylococcus aureus
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pubmed:year |
2000
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pubmed:articleTitle |
Rapid solid-phase immunoassay for detection of methicillin-resistant Staphylococcus aureus using cycling probe technology.
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pubmed:affiliation |
ID Biomedical Corp., Bothell, Washington 98011, USA.
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pubmed:publicationType |
Journal Article
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