Source:http://linkedlifedata.com/resource/pubmed/id/10875893
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2000-8-22
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| pubmed:abstractText |
The effects of adding Cu-Zn superoxide dismutase (Cu-Zn SOD) to culture medium of the murine fibroblast cell line, L-929, pretreated with UV-B (312 nm, 480 mJ/cm(2)) have been investigated. Cell injury was monitored by the release of lactate dehydrogenase (LDH) into the medium, and cell death by the trypan blue exclusion test. UV-B radiation induced cell death by apoptosis, as demonstrated by DNA fragmentation. Over the range 0.1-0.3 microm Cu-Zn SOD, a significant dose-dependent protection against cell death was obtained of the UV-B exposed cells. Cell death correlated with the amount of LDH released into the medium, and Cu-Zn SOD treatment inhibited this. Heat-denatured Cu-Zn SOD did not affect either cell viability or the release of LDH from the cells. Endogenous Cu-Zn SOD activity, monitored by chemiluminescence, decreased by 20% in UV-B-irradiated cells; the addition of 0.3 microm exogenous Cu-Zn SOD to the medium did not affect intracellular Cu-Zn SOD activity. These results establish that Cu-Zn SOD added to extracellular medium can protect cells against injury caused by UV-B exposure.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1065-6995
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:volume |
24
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
459-65
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10875893-Animals,
pubmed-meshheading:10875893-Apoptosis,
pubmed-meshheading:10875893-DNA Fragmentation,
pubmed-meshheading:10875893-Fibroblasts,
pubmed-meshheading:10875893-L Cells (Cell Line),
pubmed-meshheading:10875893-L-Lactate Dehydrogenase,
pubmed-meshheading:10875893-Luminescent Measurements,
pubmed-meshheading:10875893-Mice,
pubmed-meshheading:10875893-Superoxide Dismutase,
pubmed-meshheading:10875893-Ultraviolet Rays
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Cu-Zn superoxide dismutase inhibits lactate dehydrogenase release and protects against cell death in murine fibroblasts pretreated with ultraviolet radiation.
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| pubmed:affiliation |
Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515, Japan.
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| pubmed:publicationType |
Journal Article
|