Linked Life Data

10874015

Source:http://linkedlifedata.com/resource/pubmed/id/10874015

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-8-17
pubmed:abstractText
There are about 600 million betel quid (BQ) chewers in the world. BQ chewing is associated with increased incidence of oral cancer and submucous fibrosis. In this study, areca nut (AN) extract (200-800 microg/ml) induced the prostaglandin E(2) (PGE(2)) production by 1. 4-3.4-fold and 6-keto-PGF(1 alpha) production by 1.1-1.7-fold of gingival keratinocytes (GK), respectively, following 24 h of exposure. Exposure of GK to AN extract (>400 microg/ml) led to cell retraction and intracellular vacuoles formation. At concentrations of 800 and 1200 microg/ml, AN extract induced cell death at 21-24 and 32-52% as detected by MTT assay and cellular lactate dehydrogenase release, respectively. Interestingly, AN-induced morphological changes of GK are reversible. GK can still proliferate following exposure to AN extract. Cytotoxicity of AN extract cannot be inhibited by indomethacin (1 microM) and aspirin (50 microM), indicating that prostaglandin (PG) production is not the major factor responsible for AN cytotoxicity. PGE(2) exhibited little effect on the growth of GK at concentrations ranging from 100-1000 pg/ml. Stimulating GK production of PGs by AN extract could be due to induction of cyclooxygenase-2 (COX-2) mRNA expression and protein production. These results suggest that AN ingredients are critical in the pathogenesis of oral submucous fibrosis and oral cancer via their stimulatory effects on the PGs, COX-2 production and associated tissue inflammatory responses. AN cytotoxicity to GK is not directly mediated by COX-2 stimulation and PG production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1365-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10874015-6-Ketoprostaglandin F1 alpha, pubmed-meshheading:10874015-Animals, pubmed-meshheading:10874015-Areca, pubmed-meshheading:10874015-Blotting, Western, pubmed-meshheading:10874015-Cattle, pubmed-meshheading:10874015-Cell Death, pubmed-meshheading:10874015-Cell Size, pubmed-meshheading:10874015-Cells, Cultured, pubmed-meshheading:10874015-Cyclooxygenase 2, pubmed-meshheading:10874015-Dinoprostone, pubmed-meshheading:10874015-Dose-Response Relationship, Drug, pubmed-meshheading:10874015-Gingiva, pubmed-meshheading:10874015-Humans, pubmed-meshheading:10874015-Isoenzymes, pubmed-meshheading:10874015-Keratinocytes, pubmed-meshheading:10874015-Membrane Proteins, pubmed-meshheading:10874015-Plant Extracts, pubmed-meshheading:10874015-Plants, Medicinal, pubmed-meshheading:10874015-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:10874015-RNA, Messenger, pubmed-meshheading:10874015-Up-Regulation, pubmed-meshheading:10874015-Vacuoles
pubmed:year
2000
pubmed:articleTitle
Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes.
pubmed:affiliation
Laboratory of Dental Pharmacology and Toxicology, Graduate Institute of Clinical Dental Science, National Taiwan University, Taipei.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't