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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-7-19
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pubmed:abstractText |
A cellular protein that co-purifies with mycoviral dsRNA was isolated from the plant pathogenic fungus Helminthosporium victoriae (telomorph: Cochliobolus victoriae) infected with two viruses, the totivirus Helminthosporium victoriae 190S virus and the chrysovirus-like Helminthosporium victoriae 145S virus (Hv145SV). The cellular protein, which was, designated Hv-p68, accumulated to higher levels in virus-infected isolates compared to virus-free ones. The majority of the Hv145S dsRNAs were found in association with Hv-p68 and not packaged in virions. Hv-p68 could also be detected as a minor component of the virus capsid. Evidence is presented that Hv-p68 occurs in vivo as an octamer and that it possesses RNA-binding activities. Based on partial amino acid sequence analysis, Hv-p68 was shown to share significant sequence identity with alcohol oxidases from methylotrophic yeasts. Hv-p68 is proposed to play a role in viral RNA packaging/replication and in regulating viral pathogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-90
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10873761-Alcohol Oxidoreductases,
pubmed-meshheading:10873761-Amino Acid Sequence,
pubmed-meshheading:10873761-Capsid,
pubmed-meshheading:10873761-Centrifugation, Density Gradient,
pubmed-meshheading:10873761-Helminthosporium,
pubmed-meshheading:10873761-Microscopy, Electron,
pubmed-meshheading:10873761-Molecular Sequence Data,
pubmed-meshheading:10873761-Protein Binding,
pubmed-meshheading:10873761-Protein Structure, Quaternary,
pubmed-meshheading:10873761-RNA, Double-Stranded,
pubmed-meshheading:10873761-RNA, Viral,
pubmed-meshheading:10873761-RNA Probes,
pubmed-meshheading:10873761-RNA Viruses,
pubmed-meshheading:10873761-RNA-Binding Proteins,
pubmed-meshheading:10873761-Sequence Alignment,
pubmed-meshheading:10873761-Sequence Homology, Amino Acid,
pubmed-meshheading:10873761-Virus Assembly
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pubmed:year |
2000
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pubmed:articleTitle |
A cellular protein with an RNA-binding activity co-purifies with viral dsRNA from mycovirus-infected Helminthosporium victoriae.
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pubmed:affiliation |
Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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