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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-17
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pubmed:abstractText |
We isolated the mouse zfh-4 cDNA which is 12 kb long and capable of encoding a 3,550-amino acid protein containing four homeodomains and 22 zinc fingers including two pseudo zinc finger motifs. The mouse ZFH-4 is 51% homologous to the mouse ATBF1 and 23% to the Drosophila ZFH-2. The homeodomain and zinc finger regions are highly conserved between ZFH-4 and ATBF1 except that one zinc finger is missing in ZFH-4. Analysis of partial genomic sequences showed that the mouse zfh-4 and ATBF1 genes are similar in exon-intron organization. RT-PCR analysis of zfh-4 transcripts in adult mouse tissues showed that zfh-4 expression was low but reproducibly detectable in brain, heart, lung and muscle. In these mouse tissues, ATBF1 transcripts were poorly amplified by PCR under the conditions where zfh-4 transcripts were amplified, suggesting that the expression of zfh-4 mRNA is higher than that of ATBF1 mRNA. Other comparative analysis suggests functional similarities and dissimilarities between ZFH-4 and ATBF1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
686-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10873665-Amino Acid Motifs,
pubmed-meshheading:10873665-Amino Acid Sequence,
pubmed-meshheading:10873665-Animals,
pubmed-meshheading:10873665-Base Sequence,
pubmed-meshheading:10873665-Cloning, Molecular,
pubmed-meshheading:10873665-DNA, Complementary,
pubmed-meshheading:10873665-DNA Primers,
pubmed-meshheading:10873665-Exons,
pubmed-meshheading:10873665-Gene Expression,
pubmed-meshheading:10873665-Homeodomain Proteins,
pubmed-meshheading:10873665-Introns,
pubmed-meshheading:10873665-Mice,
pubmed-meshheading:10873665-Molecular Sequence Data,
pubmed-meshheading:10873665-RNA, Messenger,
pubmed-meshheading:10873665-Restriction Mapping,
pubmed-meshheading:10873665-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10873665-Sequence Homology, Amino Acid,
pubmed-meshheading:10873665-Zinc Fingers
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pubmed:year |
2000
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pubmed:articleTitle |
The mouse ZFH-4 protein contains four homeodomains and twenty-two zinc fingers.
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pubmed:affiliation |
Department of Biochemistry, Showa Pharmaceutical University, Machida, Tokyo, 194-8543, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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