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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-17
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pubmed:abstractText |
cdk3 has been considered to be rate-limiting for cell cycle progression of mammalian cells while its precise function remains to be elucidated. To assess cdk3 function, a cDNA coding for a cyclin-like protein (designated as ik3-1 from an interactor-1 with cdk3) was isolated with the yeast two-hybrid system using a cyclin-dependent kinase 3 (cdk3) cDNA as bait. p70(ik3-1) (a 70-kDa protein designated as p70(ik3-1)) seems to belong to the cyclin family as its C-terminal domain composed of 124 amino acids resembles the highly conserved cyclin box. Coimmunoprecipitation indicated that p70(ik3-1) binds to p35(cdk3) in vivo. The ik3-1 gene may belong to a multigene family and is highly conserved during evolution. mRNA expression of ik3-1 was low in the early G1 phase, upregulated during G1 progression, maximal at a mid-late G1 point, and declined gradually thereafter, suggesting that it may work mainly in G1 phase.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
442-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10873625-Amino Acid Sequence,
pubmed-meshheading:10873625-Animals,
pubmed-meshheading:10873625-Base Sequence,
pubmed-meshheading:10873625-Binding Sites,
pubmed-meshheading:10873625-COS Cells,
pubmed-meshheading:10873625-Carrier Proteins,
pubmed-meshheading:10873625-Cloning, Molecular,
pubmed-meshheading:10873625-Cyclin-Dependent Kinase 3,
pubmed-meshheading:10873625-Cyclin-Dependent Kinases,
pubmed-meshheading:10873625-Cyclins,
pubmed-meshheading:10873625-DNA, Complementary,
pubmed-meshheading:10873625-G1 Phase,
pubmed-meshheading:10873625-Gene Expression,
pubmed-meshheading:10873625-Mice,
pubmed-meshheading:10873625-Molecular Sequence Data,
pubmed-meshheading:10873625-Multigene Family,
pubmed-meshheading:10873625-Phosphoproteins,
pubmed-meshheading:10873625-Protein Structure, Tertiary,
pubmed-meshheading:10873625-RNA, Messenger,
pubmed-meshheading:10873625-Two-Hybrid System Techniques
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pubmed:year |
2000
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pubmed:articleTitle |
Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo.
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pubmed:affiliation |
Department of Pharmacology, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo, 160-8582, Japan. sakimatu@mc.med.keio.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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