Source:http://linkedlifedata.com/resource/pubmed/id/10871435
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-8-16
|
| pubmed:abstractText |
In this study, we hypothesize that anti-cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine CK18 was stained with patients' sera using a Western blotting. In patients with IPF, anti-CK18 antibodies were clearly demonstrated in sera by Western blotting. Then, we tried to establish an enzyme-linked immunosorbent assay (ELISA) to quantify anti-CK18 antibodies and CK18:anti-CK18 immune complexes in sera of patients with IPF. Levels of anti-human CK18 antibodies in sera of patients with IPF (0.81 +/- 0.31, mean +/- SD) measured by ELISA were significantly high compared with that of normal volunteers (0.45 +/- 0.06, p < 0.01). In addition, levels of CK18:anti-CK18 antibody complexes in patients' sera (0.64 +/- 0.35, man +/- SD) significantly increased compared with those of normal subjects (0.40 +/- 0.10, p < 0.01). These results suggest that anti-CK18 antibody and its immune complex may have played a role in the process of lung injury in IPF.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0341-2040
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
178
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
171-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10871435-Adult,
pubmed-meshheading:10871435-Aged,
pubmed-meshheading:10871435-Antigen-Antibody Complex,
pubmed-meshheading:10871435-Autoantibodies,
pubmed-meshheading:10871435-Biological Markers,
pubmed-meshheading:10871435-Blotting, Western,
pubmed-meshheading:10871435-Cell Division,
pubmed-meshheading:10871435-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10871435-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10871435-Female,
pubmed-meshheading:10871435-Humans,
pubmed-meshheading:10871435-Keratins,
pubmed-meshheading:10871435-Male,
pubmed-meshheading:10871435-Middle Aged,
pubmed-meshheading:10871435-Prognosis,
pubmed-meshheading:10871435-Pulmonary Alveoli,
pubmed-meshheading:10871435-Pulmonary Fibrosis,
pubmed-meshheading:10871435-Retrospective Studies,
pubmed-meshheading:10871435-Severity of Illness Index
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Elevation of anti-cytokeratin 18 antibody and circulating cytokeratin 18: anti-cytokeratin 18 antibody immune complexes in sera of patients with idiopathic pulmonary fibrosis.
|
| pubmed:affiliation |
First Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Multicenter Study
|