Source:http://linkedlifedata.com/resource/pubmed/id/10870964
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2000-11-3
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pubmed:abstractText |
In order to observe cellular changes caused by mutation of the tumor suppressors, APC and p53, we have generated protein expression profiles of mouse colon epithelial cells using two-dimensional electrophoresis (2-DE). Crypts, polyps and stroma were isolated from normal, multiple intestinal neoplasia (MIN) and p53-null mice, each with a C57Black/6J background, and subjected to 2-DE in two separate pH ranges (pH 3-10 and pH 6-11). No significant differences in protein expression patterns were observed between the normal, MIN and p53-null colon epithelial crypts. However, 64 proteins from the MIN polyps showed a 2-fold or greater difference in intensity that was statistically significant as assessed by the Wilcoxon rank-sum test (p < or = 0.05). Of these, calreticulin, carbonic anhydrase I and a new member of the glutathione-S-transferase theta family of proteins have so far been identified using an in-gel digestion protocol coupled with reversed-phase high performance liquid chromatography (RP-HPLC) ion-trap mass spectrometry. In addition, 38 marker proteins have been identified in a continuing effort to generate a comprehensive 2-DE database of proteins expressed by mouse colon epithelial cells (these databases are available at http://www.ludwig.edu.au/jpsl/jpslhome. html).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0173-0835
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1772-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10870964-Animals,
pubmed-meshheading:10870964-Colon,
pubmed-meshheading:10870964-Colonic Polyps,
pubmed-meshheading:10870964-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:10870964-Intestinal Neoplasms,
pubmed-meshheading:10870964-Mice,
pubmed-meshheading:10870964-Mice, Inbred C57BL,
pubmed-meshheading:10870964-Mice, Knockout,
pubmed-meshheading:10870964-Neoplasm Proteins,
pubmed-meshheading:10870964-Neoplasms, Multiple Primary,
pubmed-meshheading:10870964-Proteome,
pubmed-meshheading:10870964-Tumor Suppressor Protein p53
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pubmed:year |
2000
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pubmed:articleTitle |
Proteomic analysis of colonic crypts from normal, multiple intestinal neoplasia and p53-null mice: a comparison with colonic polyps.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Comparative Study
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