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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-7-13
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pubmed:abstractText |
Type 1 diabetes is the result of destruction of the insulin-secreting beta-cells of the pancreas by a process in which T-cells play a central role. A tyrosine phosphatase-like protein, IA-2, is a major target for autoantibodies and T-cells in the disease. In this study, we have further characterized the T-cell response to IA-2 by the generation and characterization of T-cell lines. T-cell lines responsive to IA-2 antigen were generated from 17 of 32 patients and 3 of 10 control subjects. Antigen specificity was confirmed in lines from six diabetic patients and one control individual by demonstration of responses to synthetic IA-2 peptides and epitope mapping. Five lines from diabetic patients responded to one of two peptides representing amino acids 831-850 and 841-860 of IA-2. The overlapping portion may therefore represent an immunodominant region of the molecule. The sixth patient-derived line responded to a peptide representing amino acids 751-770 of IA-2 presented by the DR 4 (DRB1*0401) allele that confers susceptibility to type 1 diabetes. Primary T-cell responses to peptides of the immunodominant region were detected in 9 of 19 (47%) type 1 diabetic patients and 16 of 22 (73%) nondiabetic siblings, consistent with this region having immunostimulatory properties. The study reports for the first time T-cell lines reactive to IA-2 from diabetic patients and defines an immunodominant region of the molecule.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunodominant Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTPRN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTPRN2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-Like Protein Tyrosine...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0012-1797
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
356-66
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10868956-Adolescent,
pubmed-meshheading:10868956-Autoantigens,
pubmed-meshheading:10868956-Cell Line,
pubmed-meshheading:10868956-Child,
pubmed-meshheading:10868956-Child, Preschool,
pubmed-meshheading:10868956-Cytoplasm,
pubmed-meshheading:10868956-Diabetes Mellitus, Type 1,
pubmed-meshheading:10868956-Epitopes,
pubmed-meshheading:10868956-Female,
pubmed-meshheading:10868956-HLA Antigens,
pubmed-meshheading:10868956-Humans,
pubmed-meshheading:10868956-Immunodominant Epitopes,
pubmed-meshheading:10868956-Infant,
pubmed-meshheading:10868956-Male,
pubmed-meshheading:10868956-Membrane Glycoproteins,
pubmed-meshheading:10868956-Membrane Proteins,
pubmed-meshheading:10868956-Peptide Fragments,
pubmed-meshheading:10868956-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:10868956-Protein Tyrosine Phosphatases,
pubmed-meshheading:10868956-Receptor-Like Protein Tyrosine Phosphatases, Class 8,
pubmed-meshheading:10868956-T-Lymphocytes
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pubmed:year |
2000
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pubmed:articleTitle |
T-cell lines reactive to an immunodominant epitope of the tyrosine phosphatase-like autoantigen IA-2 in type 1 diabetes.
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pubmed:affiliation |
Department of Medicine, Guy's, King's College and St Thomas' School of Medicine, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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