Source:http://linkedlifedata.com/resource/pubmed/id/10868949
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2000-7-6
|
pubmed:abstractText |
Mutations in genes encoding hepatocyte nuclear factor (HNF) are responsible for three of the five subtypes of maturity-onset diabetes of the young (MODY). This observation and molecular studies indicate that the HNF network is required for normal function of pancreatic beta-cells. This suggests that transcription factors involved in this complex network are candidates for genetic defects in MODY. Because the HNF-3beta gene is implicated in this network, we screened it for mutations in 21 probands of French ancestry with clinical diagnosis of MODY and early-onset type 2 diabetes. All of the five known MODY genes, HNF-4alpha, glucokinase, HNF-1alpha, HNF-1beta, and IPF1, were previously excluded as being the cause of diabetes in these families. By direct sequencing, we identified two transitions, an A-to-G at position -213 and a C-to-T at position -63 in the promoter and exon 1, respectively, of the HNF-3beta gene. A G-to-C transversion at position +32 in the intron 1 and three transitions, C-to-T at position 291, A-to-G at position 837, and G-to-A at position 1188 in the exon 3, resulting in noncoding mutations Ala97Ala, Gly279Gly, and Gln396Gln, respectively, were also identified. The allele frequencies were not significantly different between a control group and MODY probands. Familial segregation studies and linkage analysis showed that genetic variation in the HNF-3beta gene is unlikely to be the cause of early-onset type 2 diabetes in these Caucasian families.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FOXA2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 3-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0012-1797
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
49
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
306-8
|
pubmed:dateRevised |
2010-11-18
|
pubmed:meshHeading |
pubmed-meshheading:10868949-Adult,
pubmed-meshheading:10868949-Age of Onset,
pubmed-meshheading:10868949-Base Sequence,
pubmed-meshheading:10868949-DNA-Binding Proteins,
pubmed-meshheading:10868949-Diabetes Mellitus, Type 2,
pubmed-meshheading:10868949-European Continental Ancestry Group,
pubmed-meshheading:10868949-Female,
pubmed-meshheading:10868949-France,
pubmed-meshheading:10868949-Genetic Linkage,
pubmed-meshheading:10868949-Genetic Variation,
pubmed-meshheading:10868949-Hepatocyte Nuclear Factor 3-beta,
pubmed-meshheading:10868949-Humans,
pubmed-meshheading:10868949-Male,
pubmed-meshheading:10868949-Middle Aged,
pubmed-meshheading:10868949-Nuclear Proteins,
pubmed-meshheading:10868949-Transcription Factors
|
pubmed:year |
2000
|
pubmed:articleTitle |
Genetic variation in the hepatocyte nuclear factor-3beta gene (HNF3B) does not contribute to maturity-onset diabetes of the young in French Caucasians.
|
pubmed:affiliation |
Unité Propre de Recherche de l'Enseignement Supérieur Associée au Centre National de la Recherche Scientifique (CNRS UPRES-A) 8090, Institut de Biologie de Lille, France.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|