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pubmed-article:10867195pubmed:abstractTextWe generated rotavirus clones with rearrangement in vitro by serial passages of a human rotavirus strain (IGV-80-3) at high multiplicity of infection and determined nucleotide sequences of the rearranged genes from two distinct rotavirus clones, each of which possesses two rearranged genes: a common rearranged NSP1 gene and NSP3 gene with slightly different migration in polyacrylamide gel electrophoresis. Sequence analysis showed that the rearranged NSP1 and NSP3 genes had similar gene structures: concatemerization in a head to tail orientation and partial duplication of the open reading frame following the termination codon. The rearranged NSP1 gene had a direct repeat, whereas in the rearranged NSP3 gene, no such pattern was found.lld:pubmed
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pubmed-article:10867195pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:10867195pubmed:articleTitleRearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus strain.lld:pubmed
pubmed-article:10867195pubmed:affiliationDepartment of Hygiene, Sapporo Medical University School of Medicine, Japan. kkojima@sapmed.ac.jplld:pubmed
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