Source:http://linkedlifedata.com/resource/pubmed/id/10861450
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-7-13
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| pubmed:abstractText |
The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell lines express the platelet-type isoform of 12-LOX at both the mRNA and protein levels, and immunohistochemistry revealed 12-LOX expression in human prostate tumors. The enzyme was localized to the plasma membrane, cytoplasmic organelles and nucleus in non-metastatic cells (PC-3 nm) and to the cytoskeleton and nucleus in metastatic cells (DU-145). After orthotopic/intraprostatic injection of tumor cells into SCID mice, the metastatic prostate carcinoma cells (DU-145) expressed 12-LOX at a significantly higher level compared with the non-metastatic counterparts, PC-3nm. The functional involvement of 12-LOX in the metastatic process was demonstrated when DU-145 cells were pretreated in vitro with the 12-LOX inhibitors N-benzyl-N-hydroxy-5-phenylpentamide (BHPP) or baicalein, the use of which significantly inhibited lung colonization. These data suggest a potential involvement of 12-LOX in the progression of human prostate cancer.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
0020-7136
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
87
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
37-43
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:10861450-Animals,
pubmed-meshheading:10861450-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:10861450-Blood Platelets,
pubmed-meshheading:10861450-Cell Membrane,
pubmed-meshheading:10861450-Cytoplasm,
pubmed-meshheading:10861450-Cytoskeleton,
pubmed-meshheading:10861450-Disease Progression,
pubmed-meshheading:10861450-Flow Cytometry,
pubmed-meshheading:10861450-Humans,
pubmed-meshheading:10861450-Immunohistochemistry,
pubmed-meshheading:10861450-Lipoxygenase Inhibitors,
pubmed-meshheading:10861450-Male,
pubmed-meshheading:10861450-Mice,
pubmed-meshheading:10861450-Mice, SCID,
pubmed-meshheading:10861450-Microscopy, Confocal,
pubmed-meshheading:10861450-Neoplasm Invasiveness,
pubmed-meshheading:10861450-Neoplasm Transplantation,
pubmed-meshheading:10861450-Neoplasms, Experimental,
pubmed-meshheading:10861450-Phenotype,
pubmed-meshheading:10861450-Prostatic Neoplasms,
pubmed-meshheading:10861450-Protein Isoforms,
pubmed-meshheading:10861450-RNA, Messenger,
pubmed-meshheading:10861450-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10861450-Tumor Cells, Cultured
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Expression, subcellular localization and putative function of platelet-type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential.
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| pubmed:affiliation |
Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary. jtimar@oncol.hu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|