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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-9-7
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pubmed:abstractText |
Four cDNA clones named CPRD (cowpea responsive to dehydration) corresponding to genes that are responsive to dehydration were isolated using differential screening of a cDNA library prepared from 10-h dehydrated drought-tolerant cowpea (Vigna unguiculata) plants. One of the cDNA clones has a homology to 9-cis-epoxycarotenoid dioxygenase (named VuNCED1), which is supposed to be involved in abscisic acid (ABA) biosynthesis. The GST (glutathione S-transferase)-fused protein indicates a 9-cis-epoxycarotenoid dioxygenase activity, which catalyzes the cleavage of 9-cis-epoxycarotenoid. The N-terminal region of the VuNCED1 protein directed the fused sGFP (synthetic green-fluorescent protein) into the plastids of the protoplasts, indicating that the N-terminal sequence acts as a transit peptide. Both the accumulation of ABA and expression of VuNCED1 were strongly induced by drought stress in the 8-d-old cowpea plant, whereas drought stress did not trigger the expression of VuABA1 (accession no. AB030295) gene that encodes zeaxanthin epoxidase. These results indicate that the VuNCED1 cDNA encodes a 9-cis-epoxycarotenoid dioxygenase and that its product has a key role in the synthesis of ABA under drought stress.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/9-cis-epoxy-carotenoid dioxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Abscisic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Dioxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Lutein,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/zeaxanthin epoxidase
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
553-62
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10859185-Abscisic Acid,
pubmed-meshheading:10859185-Amino Acid Sequence,
pubmed-meshheading:10859185-Arabidopsis,
pubmed-meshheading:10859185-Blotting, Northern,
pubmed-meshheading:10859185-Blotting, Southern,
pubmed-meshheading:10859185-DNA, Complementary,
pubmed-meshheading:10859185-Dioxygenases,
pubmed-meshheading:10859185-Fabaceae,
pubmed-meshheading:10859185-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10859185-Gene Expression Regulation, Plant,
pubmed-meshheading:10859185-Lutein,
pubmed-meshheading:10859185-Molecular Sequence Data,
pubmed-meshheading:10859185-Oxidoreductases,
pubmed-meshheading:10859185-Oxygenases,
pubmed-meshheading:10859185-Plant Proteins,
pubmed-meshheading:10859185-Plants, Medicinal,
pubmed-meshheading:10859185-Sequence Homology, Amino Acid,
pubmed-meshheading:10859185-Water
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pubmed:year |
2000
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pubmed:articleTitle |
A stress-inducible gene for 9-cis-epoxycarotenoid dioxygenase involved in abscisic acid biosynthesis under water stress in drought-tolerant cowpea.
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pubmed:affiliation |
Laboratory of Plant Molecular Biology, RIKEN Isukuba Institute, Tsukuba, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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