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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-7-17
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pubmed:abstractText |
Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic manipulation. Our measurements are focused on a glycoprotein, contact site A (csA), as a prototype of cell-adhesion proteins. csA is expressed in aggregating cells of Dictyostelium discoideum, which are engaged in development of a multicellular organism. Adhesion between two adjacent cell surfaces involves discrete interactions characterized by an unbinding force of 23 +/- 8 pN, measured at a rupture rate of 2.5 +/- 0.5 microm s-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1465-7392
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
313-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10854320-Animals,
pubmed-meshheading:10854320-Binding Sites,
pubmed-meshheading:10854320-Calcium,
pubmed-meshheading:10854320-Cell Adhesion,
pubmed-meshheading:10854320-Cell Adhesion Molecules,
pubmed-meshheading:10854320-Cell Aggregation,
pubmed-meshheading:10854320-Cell Division,
pubmed-meshheading:10854320-Cell Membrane,
pubmed-meshheading:10854320-Chelating Agents,
pubmed-meshheading:10854320-Dictyostelium,
pubmed-meshheading:10854320-Edetic Acid,
pubmed-meshheading:10854320-Gene Expression,
pubmed-meshheading:10854320-Kinetics,
pubmed-meshheading:10854320-Microscopy, Atomic Force,
pubmed-meshheading:10854320-Mutation,
pubmed-meshheading:10854320-Phospholipids,
pubmed-meshheading:10854320-Promoter Regions, Genetic,
pubmed-meshheading:10854320-Protozoan Proteins,
pubmed-meshheading:10854320-Recombinant Fusion Proteins,
pubmed-meshheading:10854320-Spectrum Analysis,
pubmed-meshheading:10854320-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Discrete interactions in cell adhesion measured by single-molecule force spectroscopy.
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pubmed:affiliation |
Centre for Nanoscience, Ludwig Maximilians Universität München, Amalienstrabetae 54, D-80799 München, Germany. Martin.Benoit@physik.uni-muenchen.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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