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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-11-15
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pubmed:abstractText |
A new panel of steroidal cationic lipids has been synthesized for gene delivery. Using commercially available vitamin D2 (calciferol) or vitamin D3 (cholecalciferol) as hydrophobic motifs and a variety of cationic head groups as binding sites for negatively charged phosphate groups in DNA, we demonstrated that the transfection activity of the synthetic vitamin D-based cationic lipids 1d, 2d formulated with dioleoylphosphatidylethanolamine (DOPE) as a co-lipid is comparable to that of 3-(-[N-N',N'-dimethylaminoethane)carbamoyl]cholesterol (DC-Chol). These synthetic lipids are effective in transfecting a variety of cell lines. These results suggest that vitamin D-based cationic lipids are useful transfection reagents for in vitro gene transfer studies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0960-894X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-4
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10853653-3T3 Cells,
pubmed-meshheading:10853653-Animals,
pubmed-meshheading:10853653-Cations,
pubmed-meshheading:10853653-Cell Line,
pubmed-meshheading:10853653-Cholecalciferol,
pubmed-meshheading:10853653-Ergocalciferols,
pubmed-meshheading:10853653-Gene Expression,
pubmed-meshheading:10853653-Gene Targeting,
pubmed-meshheading:10853653-HeLa Cells,
pubmed-meshheading:10853653-Humans,
pubmed-meshheading:10853653-Lipids,
pubmed-meshheading:10853653-Mice,
pubmed-meshheading:10853653-Transfection,
pubmed-meshheading:10853653-Tumor Cells, Cultured,
pubmed-meshheading:10853653-Vitamin D
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pubmed:year |
2000
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pubmed:articleTitle |
Synthesis and evaluation of vitamin D-based cationic lipids for gene delivery in vitro.
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pubmed:affiliation |
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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