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rdf:type |
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lifeskim:mentions |
umls-concept:C0003320,
umls-concept:C0008169,
umls-concept:C0038975,
umls-concept:C0450240,
umls-concept:C0887917,
umls-concept:C1519595,
umls-concept:C1523987,
umls-concept:C1524063,
umls-concept:C1706210,
umls-concept:C2347567,
umls-concept:C2350483
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pubmed:issue |
1
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pubmed:dateCreated |
2000-10-16
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pubmed:abstractText |
The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 small-t antigen sequence) is the reporter. In addition to the predicted transgene transcript (1.9 kb), we identified an abundant 1.5 kb transcript which derives from an alternative splicing event that utilises a cryptic splice donor site located within the CAT gene. The native CAT open reading frame (ORF) is lost in the 1.5 kb transcript, and a western analysis has shown that protein deriving from an aberrant open reading frame is not expressed at detectable levels.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0962-8819
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
67-70
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10853270-Alternative Splicing,
pubmed-meshheading:10853270-Animals,
pubmed-meshheading:10853270-Animals, Genetically Modified,
pubmed-meshheading:10853270-Antigens, Polyomavirus Transforming,
pubmed-meshheading:10853270-Arylamine N-Acetyltransferase,
pubmed-meshheading:10853270-Base Sequence,
pubmed-meshheading:10853270-Blotting, Northern,
pubmed-meshheading:10853270-Blotting, Western,
pubmed-meshheading:10853270-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:10853270-Female,
pubmed-meshheading:10853270-Genes, Reporter,
pubmed-meshheading:10853270-Microinjections,
pubmed-meshheading:10853270-Molecular Sequence Data,
pubmed-meshheading:10853270-Open Reading Frames,
pubmed-meshheading:10853270-Rats,
pubmed-meshheading:10853270-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:10853270-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10853270-Transgenes,
pubmed-meshheading:10853270-Zygote
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pubmed:year |
2000
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pubmed:articleTitle |
Use of a cryptic splice donor site in the chloramphenicol acetyltransferase (CAT)-SV40 small-t antigen cassette generates alternative transcripts in transgenic rats.
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pubmed:affiliation |
School of Biosciences, Cardiff University, UK. smbdac@cardiff.ac.uk
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pubmed:publicationType |
Journal Article
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