Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-7-10
pubmed:abstractText
Previous results, based on inhibition of fertilization by an anti-alpha6 integrin mAb (GoH3), suggest that the alpha6beta1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L. M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095-1104). Because the egg surface tetraspanin CD9 is essential for gamete fusion (Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279-282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319-321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Suzuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321-324) and CD9 is known to associate with integrins, recent models of gamete fusion have posited that egg CD9 acts in association with alpha6beta1 in fusion (Chen, M.S., K.S. Tung, S.A. Coonrod, Y. Takahashi, D. Bigler, A. Chang, Y. Yamashita, P.W. Kincade, J.C. Herr, and J.M. White. 1999. Proc. Natl. Acad. Sci. USA. 96:11830-11835; Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279-282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319-321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Su- zuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321-324). Using eggs from cultured ovaries of mice lacking the alpha6 integrin subunit, we found that the fertilization rate, fertilization index, and sperm binding were not impaired compared with wild-type or heterozygous controls. Furthermore, a reexamination of antibody inhibition, using an assay that better simulates in vivo fertilization conditions, revealed no inhibition of fusion by the GoH3 mAb. We also found that an anti-CD9 mAb completely blocks sperm fusion with either wild-type eggs or eggs lacking alpha6beta1. Based on these results, we conclude that the alpha6beta1 integrin is not essential for sperm-egg fusion, and we suggest a new model in which CD9 acts by itself, or interacts with egg protein(s) other than alpha6beta1, to function in sperm-egg fusion.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10091056, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10459022, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10479454, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10518536, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10634790, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10634791, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-10700183, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-2154392, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-2973567, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7531445, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7600577, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7621882, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7626796, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7691492, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-7766414, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-8672529, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-8673141, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9006891, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9224676, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9402002, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9698453, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9763442, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9789327, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9794763, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9812906, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9818167, http://linkedlifedata.com/resource/pubmed/commentcorrection/10851025-9889181
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1289-96
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Normal fertilization occurs with eggs lacking the integrin alpha6beta1 and is CD9-dependent.
pubmed:affiliation
Department of Molecular and Cellular Biology, University of California Davis, Davis, California 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.