Source:http://linkedlifedata.com/resource/pubmed/id/10849430
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
34
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| pubmed:dateCreated |
2000-9-25
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| pubmed:abstractText |
Aquaporin-5 (AQP5) is a water channel protein that is selectively expressed in respiratory, salivary, and lacrimal tissues. In order to establish the tissue-specific transcriptional programs that underlie its lung- and salivary-specific expression, a 4.5-kilobase pair DNA fragment encompassing the 5'-flanking region of the rat AQP5 gene has been characterized in detail. A major transcription start site utilized in lung and salivary glands has been localized downstream of a TATAA-like motif. Transient transfection assays of -4.3- and -1.7-AQP5-luciferase constructs in AQP5-expressing lung (MLE-15) and salivary (Pa-4) cells and nonexpressing fibroblast (NIH3T3) and epithelial (HeLa) cells demonstrate preferential transcriptional enhancement of reporter activities in MLE-15 and Pa-4 cells. Transient transfection assays of a series of 5' --> 3' deletion constructs of -4.3-AQP5-luciferase suggest that a common salivary and lung enhancer is located between nucleotides -274 and -139, and a lung-specific enhancer is located between nucleotides -894 and -710. There is one putative lung-specific repressor located in the region of nucleotides -1003/-894 and a common lung and salivary repressor located at nucleotides -503/-385. Moreover, 3' --> 5' deletions up to -171 and -127 base pairs almost abolish transcriptional activation in salivary and lung cells, respectively. Together, our findings indicate that the combination of enhancer/repressor elements within the proximal 5'-flanking region of rat AQP5 gene dictates its restricted expression in both lung and salivary cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aqp5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp5 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 5,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
25
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| pubmed:volume |
275
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
26507-14
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10849430-3T3 Cells,
pubmed-meshheading:10849430-Animals,
pubmed-meshheading:10849430-Aquaporin 5,
pubmed-meshheading:10849430-Aquaporins,
pubmed-meshheading:10849430-Base Sequence,
pubmed-meshheading:10849430-Cells, Cultured,
pubmed-meshheading:10849430-Epithelial Cells,
pubmed-meshheading:10849430-Lung,
pubmed-meshheading:10849430-Male,
pubmed-meshheading:10849430-Membrane Proteins,
pubmed-meshheading:10849430-Mice,
pubmed-meshheading:10849430-Microscopy, Fluorescence,
pubmed-meshheading:10849430-Molecular Sequence Data,
pubmed-meshheading:10849430-Promoter Regions, Genetic,
pubmed-meshheading:10849430-RNA, Messenger,
pubmed-meshheading:10849430-Rats,
pubmed-meshheading:10849430-Rats, Sprague-Dawley,
pubmed-meshheading:10849430-Saliva,
pubmed-meshheading:10849430-Transcriptional Activation
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| pubmed:year |
2000
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| pubmed:articleTitle |
Differential regulation of rat aquaporin-5 promoter/enhancer activities in lung and salivary epithelial cells.
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| pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Will Rogers Institute Pulmonary Research Center, Los Angeles, CA 90033, USA. zborok@hsc.usc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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