Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-8
pubmed:abstractText
Transfusion-associated graft-versus-host disease (TA-GVHD) is a serious condition that under certain circumstances can be lethal in immunosuppressed patients. The risk of TA-GVHD can be reduced in this population by gamma irradiation (gammaRad) of blood components. gammaRad results in production of reactive oxygen species which can damage red blood cells (RBC). Tirilazad mesylate (TM) is a member of the 21-aminosteroids (Lazaroids) family and is a powerful antioxidant. We investigated the ability of TM and human plasma (which contain powerful antioxidants) to protect stored human RBC against the oxidative damage of gammaRad. Fresh intact packed RBC obtained from the normal donors, with and without autologous plasma or TM (0.05 mg mL-1 RBC), were exposed to gammaRad (50 Gy) and stored for 28 days at 4 degrees C. Oxidative damage was assessed by osmotic fragility at 65 mM NaCl concentration (expressed by percentage haemolysis in 65 mM NaCl solution) and lipid peroxidation (measured by thiobarbituric acid reactive substances, TBARS). Our results showed that storage and irradiation of untreated intact RBC increased the osmotic fragility at 65 mM NaCl concentration (65.8 +/- 1.3 vs. 51.20 +/- 0.87% haemolysis; irradiated vs. controls, respectively; P = 0.002) and lipid peroxidation (TBARS = 4.47 +/- 0. 12 vs. 3.45 +/- 0.09 microM L-1 RBC; irradiated vs. controls, respectively; P = 0.001). TM protected the intact RBC against radiation-induced haemolysis (35.8 +/- 5.0 vs. 65.8 +/- 1.3% haemolysis; treated vs. untreated irradiated RBC, respectively; P = 0.02) and lipid peroxidation (TBARS = 2.91 +/- 0.2 vs. 4.47 +/- 0.12 microM L-1 RBC; treated vs. untreated irradiated RBC, respectively; P = 0.005). Addition of autologous plasma to packed RBC significantly reduced the extent of radiation-induced haemolysis by more than six-fold (12.45 +/- 0.26 vs. 65.8 +/- 2.2% haemolysis; irradiated RBC with versus without plasma, respectively; P = 0.0001). In conclusion, these results show that irradiation and storage of blood damages RBC via oxidative processes and addition of autologous plasma and/or TM protects RBC against such damage and possibly enhances their storage and survival.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0958-7578
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-30
pubmed:dateRevised
2008-7-31
pubmed:meshHeading
pubmed-meshheading:10849383-Antioxidants, pubmed-meshheading:10849383-Blood Preservation, pubmed-meshheading:10849383-Cell Survival, pubmed-meshheading:10849383-Erythrocytes, pubmed-meshheading:10849383-Evaluation Studies as Topic, pubmed-meshheading:10849383-Free Radical Scavengers, pubmed-meshheading:10849383-Gamma Rays, pubmed-meshheading:10849383-Graft vs Host Disease, pubmed-meshheading:10849383-Hemolysis, pubmed-meshheading:10849383-Humans, pubmed-meshheading:10849383-Lipid Peroxidation, pubmed-meshheading:10849383-Osmotic Fragility, pubmed-meshheading:10849383-Oxidative Stress, pubmed-meshheading:10849383-Plasma, pubmed-meshheading:10849383-Pregnatrienes, pubmed-meshheading:10849383-Radiation Tolerance, pubmed-meshheading:10849383-Radiation-Protective Agents, pubmed-meshheading:10849383-Thiobarbituric Acid Reactive Substances
pubmed:year
2000
pubmed:articleTitle
Human plasma and tirilazad mesylate protect stored human erythrocytes against the oxidative damage of gamma-irradiation.
pubmed:affiliation
Department of Pathology, New England Medical Center, Tufts University, Boston, MA, USA.
pubmed:publicationType
Journal Article