Linked Life Data

10849108

Source:http://linkedlifedata.com/resource/pubmed/id/10849108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-8-9
pubmed:abstractText
Previous studies have demonstrated an infiltration of monocytes and increased levels of IL-1beta and TNF-alpha in some chronic inflammatory tissues. Interleukin-1beta and TNF-alpha are capable of protecting monocytes from spontaneous apoptosis and thus maintain their viability in vitro. To study the possible effects of these cytokines on the differentiation and function of recruited monocytes, a model has been developed in which monocytes isolated from human peripheral blood were differentiated into macrophages in serum in the presence or absence of IL-1beta or TNF-alpha. Monocytes cultured with IL-1beta and TNF-alpha underwent substantial changes in morphology, similar to those observed in monocytes undergoing differentiation into macrophages. The cultured cells increased in size and vacuolization and their content of acid phosphates increased 10-fold. Although they exhibited the morphological characteristics of macrophages, monocytes matured in the cytokines differed functionally from those cultured in serum in a lower expression of HLA-DR, lower ability for triggering the proliferation of allogeneic lymphocytes, higher expression of mannose receptor and greater production of superoxide and TNF-alpha. This data suggests that IL-1beta and TNF-alpha direct monocyte differentiation into macrophages with a reduced antigen-presenting and an increased pro-inflammatory factor-releasing phenotype. Elevated levels of IL-1beta and TNF-alpha in the inflammatory tissues may therefore not only prolong the survival of recruited monocytes, but maintain them in an inflammatory state.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0818-9641
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-13
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10849108-Acid Phosphatase, pubmed-meshheading:10849108-Cell Differentiation, pubmed-meshheading:10849108-Cell Size, pubmed-meshheading:10849108-Cells, Cultured, pubmed-meshheading:10849108-Cytokines, pubmed-meshheading:10849108-HLA-DR Antigens, pubmed-meshheading:10849108-Humans, pubmed-meshheading:10849108-Interleukin-1, pubmed-meshheading:10849108-Lectins, C-Type, pubmed-meshheading:10849108-Macrophages, pubmed-meshheading:10849108-Mannose-Binding Lectins, pubmed-meshheading:10849108-Microscopy, Electron, pubmed-meshheading:10849108-Monocytes, pubmed-meshheading:10849108-Receptors, Cell Surface, pubmed-meshheading:10849108-Superoxides, pubmed-meshheading:10849108-Time Factors, pubmed-meshheading:10849108-Tumor Necrosis Factor-alpha, pubmed-meshheading:10849108-Vacuoles
pubmed:year
2000
pubmed:articleTitle
Phenotypic and functional characteristics of macrophage-like cells differentiated in pro-inflammatory cytokine-containing cultures.
pubmed:affiliation
Renal Division, Division of Immunology and Rheumatology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. ffhou@public.guangzhou.gd.cn
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't