Source:http://linkedlifedata.com/resource/pubmed/id/10848832
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2000-6-26
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pubmed:abstractText |
Resveratrol, a natural product present in wine, has recently been shown to inhibit the growth of a number of cancer cell lines in vitro. In the current study, we have demonstrated that resveratrol inhibits the growth of THP-1 human monocytic leukaemia cells in a dose-dependent manner with a median effective dose of 12 microM. It did not induce differentiation of THP-1 cells and had no toxic effect on THP-1 cells that had been induced to differentiate into monocytes/macrophages by phorbol myristate acetate. A significant fraction of resveratrol-treated cells underwent apoptosis as judged by flow cytometric analysis of DNA content, DNA fragmentation and caspase-specific cleavage of poly(ADP-ribosyl) polymerase. Resveratrol treatment had no effect on the expression of Fas receptor or Fas ligand (FasL) in THP-1 cells, nor did it induce clustering of Fas receptors. In addition, THP-1 cells were resistant to activating anti-Fas antibody, and neutralizing anti-Fas and/or anti-FasL antibodies had no protective effect against resveratrol-induced inhibition of THP-1 cell growth. The effect of resveratrol on THP-1 cells was reversible after its removal from the culture medium. These results suggest that (1) resveratrol inhibits the growth of THP-1 cells, at least in part, by inducing apoptosis, (2) resveratrol-induced apoptosis of THP-1 cells is independent of the Fas/FasL signalling pathway and (3) resveratrol does not induce differentation of THP-1 cells and has no toxic effect on differentiated THP-1 cells. Thus, resveratrol may be a potential chemotherapeutic agent for the control of acute monocytic leukaemia.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Stilbenes,
http://linkedlifedata.com/resource/pubmed/chemical/resveratrol
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0007-1048
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
405-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10848832-Analysis of Variance,
pubmed-meshheading:10848832-Antigens, CD95,
pubmed-meshheading:10848832-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:10848832-Apoptosis,
pubmed-meshheading:10848832-Cell Line,
pubmed-meshheading:10848832-Fas Ligand Protein,
pubmed-meshheading:10848832-Flow Cytometry,
pubmed-meshheading:10848832-Fluorescent Antibody Technique,
pubmed-meshheading:10848832-Humans,
pubmed-meshheading:10848832-Immunoblotting,
pubmed-meshheading:10848832-Jurkat Cells,
pubmed-meshheading:10848832-Leukemia, Monocytic, Acute,
pubmed-meshheading:10848832-Membrane Glycoproteins,
pubmed-meshheading:10848832-Stilbenes
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pubmed:year |
2000
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pubmed:articleTitle |
Resveratrol induces Fas signalling-independent apoptosis in THP-1 human monocytic leukaemia cells.
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pubmed:affiliation |
Research and Medical Services, Stratton VA Medical Center, Albany, NY 12208, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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