Linked Life Data

10845695

Source:http://linkedlifedata.com/resource/pubmed/id/10845695

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-9-1
pubmed:abstractText
Microparticles were produced by spray-drying from a high molecular weight polylactide (PLA R207) for the development of long-lasting controlled release systems of vaccines, which may be designed to obviate the need for booster doses. The current investigation considered the effect of both technological parameters (inlet air temperature and spray rate of feed) and polymeric solutions (polymer concentration and nature of organic solvents) on characteristics of microparticles (morphology, size and antigen loading) containing a water-soluble model antigen (bovine serum albumin, BSA). Following parameters chosen, microparticles were characterized by a mean size from 3.08 +/- 0.06 to 9.43 +/- 0.26 microm and a BSA loading from 2.45 +/- 0.13 to 18.20 +/- 2.25% (w/w). The BSA release rate from microparticles varied from 11.17 +/- 2.20 to 92.60 +/- 3.46% in 24 h. The modification of the inlet temperature, the spray-rate of feed or the use of a mixture of dichloromethane/chloroform (DCM/CFM) instead of DCM alone resulted in the modification of the BSA burst release. This burst release was followed by a BSA release rate slower for microparticles with a low BSA loading. Moreover, the increase of the R207 concentration resulted in a decrease of the BSA release rate while the burst release was not modified. SDS-PAGE electrophoresis and isoelectric focusing analyses of the BSA released from microparticles confirmed the preservation of its physicochemical characteristics. Together, results showed that the spray-dried microparticles loaded with hydrophilic antigen could be used as a potential delivery system for the long-lasting controlled release of vaccines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Parameters influencing the antigen release from spray-dried poly(DL-lactide) microparticles.
pubmed:affiliation
Laboratoire de Pharmacie Galénique, Industrielle et Officinale, Ecole de Pharmacie, Université Catholique de Louvain, Brussels, Belgium. benoit.baras@pasteur-lille.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't