Linked Life Data

10844655

Source:http://linkedlifedata.com/resource/pubmed/id/10844655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-8-29
pubmed:abstractText
The aglU gene of Myxococcus xanthus encodes a protein similar to Het-E1 (vegetative incompatibility) from Podospora anserina, acylaminoacyl-peptidase from Bacillus subtilis, and TolB from Escherichia coli. These proteins all have evenly spaced SPDG repeats that are characteristic of a larger motif called the WD-repeat. The WD-repeat is predicted to form a beta-propeller structure that mediates the assembly of heteromeric protein complexes. AglU has a consensus lipoprotein attachment motif that includes a type II signal sequence followed by a cysteine residue. This suggests that AglU is matured, then attached to the outer membrane via fatty acid acylation at this Cys. Cells carrying a mutation in aglU are blocked in adventurous gliding and can swarm only if cells are in contact with one another. When starved of nutrients, the aglU mutant aggregates and forms multicellular fruiting bodies like the wild-type strain, but is unable to produce heat-resistant spores. This suggests that adventurous gliding motility, per se, is not required for development, but that AglU is essential for a terminal step of spore differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
662-78
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed-meshheading:10844655-Amino Acid Sequence, pubmed-meshheading:10844655-Bacillus subtilis, pubmed-meshheading:10844655-Bacterial Proteins, pubmed-meshheading:10844655-Base Sequence, pubmed-meshheading:10844655-DNA Primers, pubmed-meshheading:10844655-Escherichia coli, pubmed-meshheading:10844655-Escherichia coli Proteins, pubmed-meshheading:10844655-Fungal Proteins, pubmed-meshheading:10844655-GTP-Binding Proteins, pubmed-meshheading:10844655-Genetic Complementation Test, pubmed-meshheading:10844655-Lipoproteins, pubmed-meshheading:10844655-Molecular Sequence Data, pubmed-meshheading:10844655-Movement, pubmed-meshheading:10844655-Myxococcus xanthus, pubmed-meshheading:10844655-Peptide Hydrolases, pubmed-meshheading:10844655-Periplasmic Proteins, pubmed-meshheading:10844655-Phenotype, pubmed-meshheading:10844655-Repetitive Sequences, Amino Acid, pubmed-meshheading:10844655-Sequence Alignment, pubmed-meshheading:10844655-Sequence Homology, Amino Acid, pubmed-meshheading:10844655-Spores, Bacterial
pubmed:year
2000
pubmed:articleTitle
AglU, a protein required for gliding motility and spore maturation of Myxococcus xanthus, is related to WD-repeat proteins.
pubmed:affiliation
Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83843-3052, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.