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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2000-10-10
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pubmed:abstractText |
We previously demonstrated that a member of the Hedgehog gene family, Indian hedgehog (Ihh), is expressed in the visceral endoderm of EC and ES cell embryoid bodies and mouse embryos. Overexpression studies suggested that Ihh was involved in visceral endoderm differentiation. We now provide evidence for a Hh response in the embryoid body core and in the mesothelial layer of the visceral yolk sac. We also demonstrate that treatment of ES embryoid bodies with the Hh antagonists cAMP and forskolin results in downregulation of the Hh response and altered embryoid body differentiation. The outer endoderm layer undergoes a transition to parietal endoderm while formation of an embryonic ectoderm layer surrounding a cavity is inhibited. These treatments also result in a decrease in the expression of markers for the mesoderm derivatives, blood and endothelial cells. We present a model to explain how Ihh and BMP signaling may regulate extraembryonic endoderm and embryonic ectoderm differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 4,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/GLI3 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/GLI3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gli protein,
http://linkedlifedata.com/resource/pubmed/chemical/Gli2 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Gli3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bmp4 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/patched receptors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0925-4773
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-32
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10842064-Animals,
pubmed-meshheading:10842064-Biological Markers,
pubmed-meshheading:10842064-Bone Morphogenetic Protein 2,
pubmed-meshheading:10842064-Bone Morphogenetic Protein 4,
pubmed-meshheading:10842064-Bone Morphogenetic Proteins,
pubmed-meshheading:10842064-Cell Differentiation,
pubmed-meshheading:10842064-Cyclic AMP,
pubmed-meshheading:10842064-DNA-Binding Proteins,
pubmed-meshheading:10842064-Ectoderm,
pubmed-meshheading:10842064-Embryo, Mammalian,
pubmed-meshheading:10842064-Embryonic Induction,
pubmed-meshheading:10842064-Endoderm,
pubmed-meshheading:10842064-Endothelium,
pubmed-meshheading:10842064-Fetal Blood,
pubmed-meshheading:10842064-Forskolin,
pubmed-meshheading:10842064-Gene Expression Regulation, Developmental,
pubmed-meshheading:10842064-Hedgehog Proteins,
pubmed-meshheading:10842064-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10842064-Kruppel-Like Transcription Factors,
pubmed-meshheading:10842064-Membrane Proteins,
pubmed-meshheading:10842064-Mice,
pubmed-meshheading:10842064-Nerve Tissue Proteins,
pubmed-meshheading:10842064-Oncogene Proteins,
pubmed-meshheading:10842064-Proteins,
pubmed-meshheading:10842064-Receptors, Cell Surface,
pubmed-meshheading:10842064-Repressor Proteins,
pubmed-meshheading:10842064-Signal Transduction,
pubmed-meshheading:10842064-Trans-Activators,
pubmed-meshheading:10842064-Transcription Factors,
pubmed-meshheading:10842064-Transforming Growth Factor beta,
pubmed-meshheading:10842064-Xenopus Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Indian hedgehog signaling in extraembryonic endoderm and ectoderm differentiation in ES embryoid bodies.
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pubmed:affiliation |
Biology Department, Wesleyan University, Middletown, CT 06459-0170, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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