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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2000-7-13
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pubmed:databankReference |
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pubmed:abstractText |
A hypothetical gene with similarity to the ispD gene of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF of 909 bp specifies a protein of 302 amino acid residues. The cognate chromosomal gene consists of 2,071 bp and comprises 11 introns with a size range of 78-202 bp. A fragment comprising amino acid residues 76-302 was expressed in a recombinant E. coli strain. The protein was purified to homogeneity and was shown to catalyze the formation of 4-diphosphocytidyl-2C-methyl-d-erythritol from 2C-methyl-d-erythritol 4-phosphate with a specific activity of 67 micromol small middle dotmin(-1) mg(-1). The Michaelis constants for 4-diphosphocytidyl-2C-methyl-d-erythritol and CTP were 500 microM and 114 microM, respectively.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10222052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10338008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10428488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10477522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10518523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10655484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-10694574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-3661981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-4552986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-8240251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-8980472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9009212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9371765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9380681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9482845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9482846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9701598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9707569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10841550-9751645
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6451-6
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana.
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pubmed:affiliation |
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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