Linked Life Data

10837354

Source:http://linkedlifedata.com/resource/pubmed/id/10837354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-7-18
pubmed:abstractText
We determined the role of the multidrug resistance (MDR1) gene product, P-glycoprotein (PGP), in the secretion of aldosterone by the adrenal cell line NCI-H295. Aldosterone secretion is significantly decreased by the PGP inhibitors verapamil, cyclosporin A (CSA), PSC-833, and vinblastine. Aldosterone inhibits the efflux of the PGP substrate rhodamine 123 from NCI-H295 cells and from human mesangial cells (expressing PGP). CSA, verapamil, and the monoclonal antibody UIC2 significantly decreased the efflux of fluorescein-labeled (FL)-aldosterone microinjected into NCI-H295 cells. In MCF-7/VP cells, expressing multidrug resistance-associated protein (MRP) but not PGP, and in the parental cell line MCF7 (expressing no MRP and no PGP), the efflux of microinjected FL-aldosterone was slow. In BC19/3 cells (MCF7 cells transfected with MDR1), the efflux of FL-aldosterone was rapid and it was inhibited by verapamil, indicating that transfection with MDR1 cDNA confers the ability to transport FL-aldosterone. These results strongly indicate that PGP plays a role in the secretion of aldosterone by NCI-H295 cells and in other cells expressing MDR1, including normal adrenal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1256-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10837354-Adrenal Glands, pubmed-meshheading:10837354-Aldosterone, pubmed-meshheading:10837354-Breast Neoplasms, pubmed-meshheading:10837354-Cell Line, pubmed-meshheading:10837354-Cell Membrane, pubmed-meshheading:10837354-Drug Resistance, Multiple, pubmed-meshheading:10837354-Etoposide, pubmed-meshheading:10837354-Female, pubmed-meshheading:10837354-Glomerular Mesangium, pubmed-meshheading:10837354-Humans, pubmed-meshheading:10837354-Oligodeoxyribonucleotides, Antisense, pubmed-meshheading:10837354-P-Glycoprotein, pubmed-meshheading:10837354-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10837354-Rhodamine 123, pubmed-meshheading:10837354-Transcription, Genetic, pubmed-meshheading:10837354-Transfection, pubmed-meshheading:10837354-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Role of multidrug resistance P-glycoprotein in the secretion of aldosterone by human adrenal NCI-H295 cells.
pubmed:affiliation
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't