Source:http://linkedlifedata.com/resource/pubmed/id/10837348
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2000-7-18
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| pubmed:databankReference | |
| pubmed:abstractText |
We screened rat brain cDNA libraries and used 5' rapid amplification of cDNA ends to clone two electrogenic Na(+)-HCO(-)(3) cotransporter (NBC) isoforms from rat brain (rb1NBC and rb2NBC). At the amino acid level, one clone (rb1NBC) is 96% identical to human pancreas NBC. The other clone (rb2NBC) is identical to rb1NBC except for 61 unique COOH-terminal amino acids, the result of a 97-bp deletion near the 3' end of the open-reading frame. Using RT-PCR, we confirmed that mRNA from rat brain contains this 97-bp deletion. Furthermore, we generated rabbit polyclonal antibodies that distinguish between the unique COOH-termini of rb1NBC (alpharb1NBC) and rb2NBC (alpharb2NBC). alpharb1NBC labels an approximately 130-kDa protein predominantly from kidney, and alpharb2NBC labels an approximately 130-kDa protein predominantly from brain. alpharb2NBC labels a protein that is more highly expressed in cortical neurons than astrocytes cultured from rat brain; alpharb1NBC exhibits the opposite pattern. In expression studies, applying 1.5% CO(2)/10 mM HCO(-)(3) to Xenopus oocytes injected with rb2NBC cRNA causes 1) pH(i) to recover from the initial CO(2)-induced acidification and 2) the cell to hyperpolarize. Subsequently, removing external Na(+) reverses the pH(i) increase and elicits a rapid depolarization. In the presence of 450 microM DIDS, removing external Na(+) has no effect on pH(i) and elicits a small hyperpolarization. The rate of the pH(i) decrease elicited by removing Na(+) is insensitive to removing external Cl(-). Thus rb2NBC is a DIDS-sensitive, electrogenic NBC that is predominantly expressed in brain of at least rat.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Bicarbonate Symporters
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0363-6143
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
278
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
C1200-11
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10837348-Amino Acid Sequence,
pubmed-meshheading:10837348-Animals,
pubmed-meshheading:10837348-Antibodies,
pubmed-meshheading:10837348-Brain,
pubmed-meshheading:10837348-Carrier Proteins,
pubmed-meshheading:10837348-Cloning, Molecular,
pubmed-meshheading:10837348-Female,
pubmed-meshheading:10837348-Gene Library,
pubmed-meshheading:10837348-Humans,
pubmed-meshheading:10837348-Membrane Potentials,
pubmed-meshheading:10837348-Molecular Sequence Data,
pubmed-meshheading:10837348-Oocytes,
pubmed-meshheading:10837348-Protein Isoforms,
pubmed-meshheading:10837348-RNA, Messenger,
pubmed-meshheading:10837348-Rabbits,
pubmed-meshheading:10837348-Rats,
pubmed-meshheading:10837348-Recombinant Proteins,
pubmed-meshheading:10837348-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10837348-Sequence Alignment,
pubmed-meshheading:10837348-Sequence Deletion,
pubmed-meshheading:10837348-Sequence Homology, Amino Acid,
pubmed-meshheading:10837348-Sodium-Bicarbonate Symporters,
pubmed-meshheading:10837348-Xenopus laevis
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| pubmed:year |
2000
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| pubmed:articleTitle |
An electrogenic Na(+)-HCO(-)(3) cotransporter (NBC) with a novel COOH-terminus, cloned from rat brain.
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| pubmed:affiliation |
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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