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rdf:type |
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lifeskim:mentions |
umls-concept:C0003873,
umls-concept:C0004561,
umls-concept:C0005953,
umls-concept:C0030705,
umls-concept:C0038250,
umls-concept:C0183683,
umls-concept:C0205359,
umls-concept:C0229664,
umls-concept:C0237401,
umls-concept:C0344211,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1332710,
umls-concept:C1510411,
umls-concept:C1521721
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|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2000-7-31
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|
pubmed:abstractText |
We show that bone marrow (BM) CD34+ progenitor cells from rheumatoid arthritis (RA) patients have the capacity to support spontaneous transformation of peripheral blood B cells. CD34+ cells purified from BM blood from eight RA patients and eight osteoarthritis (OA) patients were expanded with granulocyte/macrophage colony stimulating factor (GM-CSF) for 4-6 weeks. GM-CSF-stimulated BM CD34+ cells from three of eight RA patients, but none from seven OA patients, gave rise to spontaneous transformation of highly purified B cells of Epstein-Barr virus (EBV)-seronegative healthy donors. GM-CSF-stimulated BM CD34+ cells from four of six RA patients and from one of four OA patients also supported the spontaneous transformation of peripheral blood B cells from EBV-seropositive healthy donors. All the transformed B cell lines were positive for EBV-DNA as determined by PCR. Neither GM-CSF-stimulated BM CD34+ cells alone nor highly purified B cells alone gave rise to spontaneously transformed B cell lines. These results suggest that the capacity of BM CD34+ cells to support survival of B cells might contribute to the pathogenesis of RA by sustaining abnormal B cell responses.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0172-8172
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
19
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|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
153-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10836526-Antigens, CD14,
pubmed-meshheading:10836526-Antigens, CD19,
pubmed-meshheading:10836526-Antigens, CD34,
pubmed-meshheading:10836526-Arthritis, Rheumatoid,
pubmed-meshheading:10836526-B-Lymphocytes,
pubmed-meshheading:10836526-Bone Marrow,
pubmed-meshheading:10836526-Cell Count,
pubmed-meshheading:10836526-Cell Size,
pubmed-meshheading:10836526-Cells, Cultured,
pubmed-meshheading:10836526-Flow Cytometry,
pubmed-meshheading:10836526-Fluorescent Antibody Technique,
pubmed-meshheading:10836526-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10836526-HLA-DR Antigens,
pubmed-meshheading:10836526-Herpesvirus 4, Human,
pubmed-meshheading:10836526-Humans,
pubmed-meshheading:10836526-Lymphocyte Activation,
pubmed-meshheading:10836526-Osteoarthritis,
pubmed-meshheading:10836526-Stem Cell Factor,
pubmed-meshheading:10836526-Stem Cells
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|
pubmed:year |
2000
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|
pubmed:articleTitle |
Bone marrow CD34+ progenitor cells from rheumatoid arthritis patients support spontaneous transformation of peripheral blood B cells from healthy individuals.
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pubmed:affiliation |
Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan. shunsei@med.teikyo-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
