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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-9-25
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pubmed:abstractText |
Gene therapy applications of retroviral vectors derived from C-type retroviruses have been limited to introducing genes into dividing target cells. Here, we report genetically engineered C-type retroviral vectors derived from spleen necrosis virus (SNV), which are capable of infecting nondividing cells. This has been achieved by introducing a nuclear localization signal (NLS) sequence into the matrix protein (MA) of SNV by site-directed mutagenesis. This increased the efficiency of infecting nondividing cells and was sufficient to endow the virus with the capability to efficiently infect growth-arrested human T lymphocytes and quiescent primary monocyte-derived macrophages. We demonstrate that this vector actively penetrates the nucleus of a target cell, and has potential use as a gene therapy vector to transfer genes into nondividing cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1087-0156
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10835599-Amino Acid Sequence,
pubmed-meshheading:10835599-Animals,
pubmed-meshheading:10835599-Blotting, Southern,
pubmed-meshheading:10835599-Cell Division,
pubmed-meshheading:10835599-Cell Line,
pubmed-meshheading:10835599-Cell Nucleus,
pubmed-meshheading:10835599-Dogs,
pubmed-meshheading:10835599-Extracellular Matrix Proteins,
pubmed-meshheading:10835599-Flow Cytometry,
pubmed-meshheading:10835599-Gammaretrovirus,
pubmed-meshheading:10835599-Gene Products, gag,
pubmed-meshheading:10835599-Gene Transfer Techniques,
pubmed-meshheading:10835599-Genetic Vectors,
pubmed-meshheading:10835599-HeLa Cells,
pubmed-meshheading:10835599-Humans,
pubmed-meshheading:10835599-Jurkat Cells,
pubmed-meshheading:10835599-Macrophages,
pubmed-meshheading:10835599-Molecular Sequence Data,
pubmed-meshheading:10835599-Monocytes,
pubmed-meshheading:10835599-Mutagenesis, Site-Directed,
pubmed-meshheading:10835599-Necrosis,
pubmed-meshheading:10835599-Nuclear Localization Signals,
pubmed-meshheading:10835599-Plasmids,
pubmed-meshheading:10835599-Spleen,
pubmed-meshheading:10835599-T-Lymphocytes,
pubmed-meshheading:10835599-Transfection,
pubmed-meshheading:10835599-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Spleen necrosis virus-derived C-type retroviral vectors for gene transfer to quiescent cells.
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pubmed:affiliation |
The Dorrance H. Hamilton Laboratories, Thomas Jefferson University, Division of Infectious Diseases, Center for Human Virology, 1020 Locust Street, Suite 329, Philadelphia, PA 19107, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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