Linked Life Data

10834956

Source:http://linkedlifedata.com/resource/pubmed/id/10834956

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-7-26
pubmed:abstractText
We have designed a universal PCR capable of amplifying a portion of the 16S rRNA gene of eubacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecium, Enterococcus faecalis, Mycobacterium tuberculosis, Legionella pneumophila, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, Proteus mirabilis, Haemophilus influenzae, and Neisseria meningitidis. The sizes of the amplified products from various bacteria were the same (996 bp), but the restriction patterns of most PCR products generated by HaeIII digestion were different. PCR products from S. aureus and S. epidermidis could not be digested by HaeIII but yielded different patterns when they were digested with MnlI. PCR products from S. pneumoniae, E. faecium, and E. faecalis yielded the same HaeIII digestion pattern but could be differentiated by AluI digestion. PCR products from E. coli, K. pneumoniae, S. marcescens, and E. cloacae also had the same HaeIII digestion pattern but had different patterns when digested with DdeI or BstBI. This universal PCR could detect as few as 10 E. coli or 250 S. aureus organisms. Compared with culture, the sensitivity of this universal PCR for detection and identification of bacteria directly from 150 cerebrospinal fluids was 92.3%. These results suggest that this universal PCR coupled with restriction enzyme analysis can be used to detect and identify bacterial pathogens in clinical specimens.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-1445517, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-2095137, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-2413450, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-3538319, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-6365957, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-6828812, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-6863507, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-7512093, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-7751363, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-7852565, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-8253962, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-8349758, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-9672133, http://linkedlifedata.com/resource/pubmed/commentcorrection/10834956-9817881
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2076-80
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid.
pubmed:affiliation
Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan, Republic of China. JJL@NDMCTSGH.EDU.TW
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't