Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2000-8-9
pubmed:abstractText
Primary cultures of rat cortical astrocytes express phospholipase D (PLD) isoforms 1 and 2 as determined by RT-PCR and Western blot. Basal PLD activity was strongly (10-fold) increased by 4beta-phorbol-12beta,13alpha-dibutyrate (PDB) (EC(50): 56 nM), an effect which was inhibited by Ro 31-8220 (0.1-1 microM), an inhibitor of protein kinase C (PKC), and by brefeldin A (10-100 microg/ml), an inhibitor of ADP-ribosylating factor (ARF) activation. Pretreatment of the cultures with Clostridium difficile toxin B-10463 (0.1-1 ng/ml), which inactivates small G proteins of the Rho family, led to a breakdown of the astroglial cytoskeleton; concomitantly, PLD activation by PDB was reduced by up to 50%. In contrast, inactivation of proteins of the Ras family by Clostridium sordellii lethal toxin 1522 did not affect PLD activation. In parallel experiments, serum-induced PLD activation was sensitive to brefeldin A, but not to Ro 31-8220 and not to clostridial toxins. We conclude that, in astrocytes, the PLD isoform which is activated by phorbol ester requires PKC, ARF and Rho proteins for full activity and probably represents PLD1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ADP-Ribosylation Factors, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins, http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Ro 31-8220, http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine, http://linkedlifedata.com/resource/pubmed/chemical/lethal toxin LT, Clostridium..., http://linkedlifedata.com/resource/pubmed/chemical/phospholipase D1, http://linkedlifedata.com/resource/pubmed/chemical/phospholipase D2, http://linkedlifedata.com/resource/pubmed/chemical/rho GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/toxB protein, Clostridium difficile
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
1485
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-62
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10832096-ADP-Ribosylation Factors, pubmed-meshheading:10832096-Animals, pubmed-meshheading:10832096-Astrocytes, pubmed-meshheading:10832096-Bacterial Proteins, pubmed-meshheading:10832096-Bacterial Toxins, pubmed-meshheading:10832096-Brefeldin A, pubmed-meshheading:10832096-Cells, Cultured, pubmed-meshheading:10832096-Enzyme Activation, pubmed-meshheading:10832096-Enzyme Inhibitors, pubmed-meshheading:10832096-Indoles, pubmed-meshheading:10832096-Phorbol Esters, pubmed-meshheading:10832096-Phospholipase D, pubmed-meshheading:10832096-Protein Kinase C, pubmed-meshheading:10832096-Protein Synthesis Inhibitors, pubmed-meshheading:10832096-Rats, pubmed-meshheading:10832096-Rats, Sprague-Dawley, pubmed-meshheading:10832096-Serum Albumin, Bovine, pubmed-meshheading:10832096-rho GTP-Binding Proteins
pubmed:year
2000
pubmed:articleTitle
Activation of astroglial phospholipase D activity by phorbol ester involves ARF and Rho proteins.
pubmed:affiliation
Department of Pharmacology, University of Mainz, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't