Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-8-18
pubmed:abstractText
Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. To develop methods to investigate the full extent of microbial diversity, we used a bacterial artificial chromosome (BAC) vector to construct libraries of genomic DNA isolated directly from soil (termed metagenomic libraries). To date, we have constructed two such libraries, which contain more than 1 Gbp of DNA. Phylogenetic analysis of 16S rRNA gene sequences recovered from one of the libraries indicates that the BAC libraries contain DNA from a wide diversity of microbial phyla, including sequences from diverse taxa such as the low-G+C, gram-positive Acidobacterium, Cytophagales, and Proteobacteria. Initial screening of the libraries in Escherichia coli identified several clones that express heterologous genes from the inserts, confirming that the BAC vector can be used to maintain, express, and analyze environmental DNA. The phenotypes expressed by these clones include antibacterial, lipase, amylase, nuclease, and hemolytic activities. Metagenomic libraries are a powerful tool for exploring soil microbial diversity, providing access to the genetic information of uncultured soil microorganisms. Such libraries will be the basis of new initiatives to conduct genomic studies that link phylogenetic and functional information about the microbiota of environments dominated by microorganisms that are refractory to cultivation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-10339608, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-10347042, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-10473393, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-1528894, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-1612428, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-1691827, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-2330053, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-2952030, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-2999794, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-3904603, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-7535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-7630383, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-7800481, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8550487, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8593035, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8597544, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8661051, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8663938, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-8990199, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9293013, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9393700, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9415872, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9524234, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9560243, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9618454, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9650576, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9733676, http://linkedlifedata.com/resource/pubmed/commentcorrection/10831436-9818143
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2541-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10831436-Amino Acid Sequence, pubmed-meshheading:10831436-Amylases, pubmed-meshheading:10831436-Bacteria, pubmed-meshheading:10831436-Chromosomes, Bacterial, pubmed-meshheading:10831436-Cloning, Molecular, pubmed-meshheading:10831436-DNA, Bacterial, pubmed-meshheading:10831436-Deoxyribonucleases, pubmed-meshheading:10831436-Ecosystem, pubmed-meshheading:10831436-Genes, rRNA, pubmed-meshheading:10831436-Genome, Bacterial, pubmed-meshheading:10831436-Genomic Library, pubmed-meshheading:10831436-Hemolysis, pubmed-meshheading:10831436-Lipase, pubmed-meshheading:10831436-Molecular Sequence Data, pubmed-meshheading:10831436-Open Reading Frames, pubmed-meshheading:10831436-Phylogeny, pubmed-meshheading:10831436-RNA, Ribosomal, 16S, pubmed-meshheading:10831436-Soil Microbiology
pubmed:year
2000
pubmed:articleTitle
Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms.
pubmed:affiliation
Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.
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