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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-7-6
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pubmed:abstractText |
The putative Rab3 effector RIM (Rab3-interacting molecule) was detected by Northern blotting, RT-PCR and Western blotting in native pancreatic beta-cells as well as in the derived cell lines INS-1E and HIT-T15. RIM was localized on the plasma membrane of INS-1E cells and beta-cells. An involvement of RIM in insulin exocytosis was indicated by transfection experiments of INS-1E cells with the Rab3 binding domain of RIM. This domain enhanced glucose-stimulated secretion in intact cells and Ca(2+)-stimulated exocytosis in permeabilized cells. Co-expression of Rab3A reversed the effect of RIM on exocytosis. These results suggest an implication of RIM in the control of insulin secretion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RIMS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Rim protein, mammalian,
http://linkedlifedata.com/resource/pubmed/chemical/Rim1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Rims1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/rab3 GTP-Binding Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
474
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
66-70
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10828453-Animals,
pubmed-meshheading:10828453-Cell Line,
pubmed-meshheading:10828453-Cell Membrane,
pubmed-meshheading:10828453-Cricetinae,
pubmed-meshheading:10828453-Exocytosis,
pubmed-meshheading:10828453-GTP-Binding Proteins,
pubmed-meshheading:10828453-Gene Expression,
pubmed-meshheading:10828453-Humans,
pubmed-meshheading:10828453-Insulin,
pubmed-meshheading:10828453-Insulinoma,
pubmed-meshheading:10828453-Islets of Langerhans,
pubmed-meshheading:10828453-Mice,
pubmed-meshheading:10828453-Nerve Tissue Proteins,
pubmed-meshheading:10828453-Pancreatic Neoplasms,
pubmed-meshheading:10828453-Rats,
pubmed-meshheading:10828453-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10828453-Tumor Cells, Cultured,
pubmed-meshheading:10828453-rab3 GTP-Binding Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis.
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pubmed:affiliation |
Division de Biochimie Clinique, Centre Médical Universitaire, Departement de Médecine Interne, Université de Genevé, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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