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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2000-6-29
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pubmed:abstractText |
Vascular endothelial growth factor (VEGF) is highly expressed in vascular remodeling processes and accelerates reendothelialization after mechanical denudation. Two VEGF tyrosine kinase receptors have been reported-fms-like-tyrosine kinase-1 (Flt-1) and kinase domain region (KDR). Little is known about the regulation of the expression of these receptors after vascular injury. Herein, we have analyzed the expression of Flt-1 after mechanical denudation of primary cultures of endothelial cells, which has been considered a useful in vitro model to study endothelium responses to vascular injury. After denudation, the Flt-1 protein and mRNA levels are clearly up-regulated, and transient transfection experiments showed a strong induction of the flt-1 promoter-dependent transcription. Analysis of the flt-1 promoter sequence revealed the presence of a putative binding site for the early growth response factor-1 (Egr-1) at positions -24 to -16. Electrophoretic mobility shift and supershift assays showed that Egr-1 was able to bind to this DNA sequence, and cotransfection of the flt-1 promoter reporter plasmid with an Egr-1 expression vector resulted in enhancement of its transcriptional activity. Furthermore, the mutation of the Egr-1 binding site markedly reduced the denudation-induced flt-1 promoter activity. These data demonstrate that Flt-1 is up-regulated after endothelial denudation and that Egr-1 plays a relevant role in this process.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EGR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3387-95
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10828020-Binding Sites,
pubmed-meshheading:10828020-Cell Nucleus,
pubmed-meshheading:10828020-Cells, Cultured,
pubmed-meshheading:10828020-DNA-Binding Proteins,
pubmed-meshheading:10828020-Early Growth Response Protein 1,
pubmed-meshheading:10828020-Endothelium, Vascular,
pubmed-meshheading:10828020-Humans,
pubmed-meshheading:10828020-Immediate-Early Proteins,
pubmed-meshheading:10828020-Kinetics,
pubmed-meshheading:10828020-Luciferases,
pubmed-meshheading:10828020-Muscle, Smooth, Vascular,
pubmed-meshheading:10828020-Promoter Regions, Genetic,
pubmed-meshheading:10828020-Proto-Oncogene Proteins,
pubmed-meshheading:10828020-RNA, Messenger,
pubmed-meshheading:10828020-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:10828020-Receptors, Growth Factor,
pubmed-meshheading:10828020-Recombinant Proteins,
pubmed-meshheading:10828020-Transcription, Genetic,
pubmed-meshheading:10828020-Transcription Factors,
pubmed-meshheading:10828020-Transfection,
pubmed-meshheading:10828020-Umbilical Veins,
pubmed-meshheading:10828020-Up-Regulation,
pubmed-meshheading:10828020-Vascular Endothelial Growth Factor Receptor-1
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pubmed:year |
2000
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pubmed:articleTitle |
Up-regulation of vascular endothelial growth factor receptor Flt-1 after endothelial denudation: role of transcription factor Egr-1.
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pubmed:affiliation |
Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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