Linked Life Data

10825133

Source:http://linkedlifedata.com/resource/pubmed/id/10825133

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2000-6-13
pubmed:abstractText
The effects of exposure to high and very low fluence alpha-particles on the G1 checkpoint were investigated in human diploid fibroblasts irradiated and released from density-inhibited confluent cultures by the use of the cumulative labeling index method. Transient and permanent arrests in G1 occurred in fibroblast populations exposed to mean doses as low as 1 cGy, suggesting that nontraversed bystander cells may contribute to the low dose response. In cells exposed to high fluences, the G1 checkpoint is at least as extensive as in gamma-irradiated cells. In contrast to gamma-irradiated cells, neither repair of potentially lethal damage nor a reduction in the fraction of cells transiently or permanently arrested in G1 were observed in cells held in confluence for 6 h after alpha-particle irradiation. Studies with isogenic wild-type, p53-/-, and p21Waf1-/- mouse embryo fibroblasts exposed to either gamma or alpha-particle radiation revealed a total lack of G1 arrest in either p53-/- or p21waf1-/- cells, indicating that the G1 checkpoint in wild-type cells is p53-dependent and that p21Wf1 fully mediates the role of p53 in its induction. In contrast to human cells, mouse embryo fibroblasts do not undergo a permanent G1 arrest. Except under conditions favoring potentially lethal damage repair, a comparable expression pattern of p53, p21Waf1, and other cell cycle-regulated proteins (pRb, p34cdc2, and cyclin B1) was observed in alpha-particle or gamma-irradiated human fibroblasts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2623-31
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10825133-Alpha Particles, pubmed-meshheading:10825133-Animals, pubmed-meshheading:10825133-CDC2 Protein Kinase, pubmed-meshheading:10825133-Cell Line, pubmed-meshheading:10825133-Cell Survival, pubmed-meshheading:10825133-Cyclin B, pubmed-meshheading:10825133-Cyclin B1, pubmed-meshheading:10825133-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:10825133-Cyclins, pubmed-meshheading:10825133-DNA Replication, pubmed-meshheading:10825133-Fibroblasts, pubmed-meshheading:10825133-G1 Phase, pubmed-meshheading:10825133-Gamma Rays, pubmed-meshheading:10825133-Gene Expression Regulation, pubmed-meshheading:10825133-Humans, pubmed-meshheading:10825133-Mice, pubmed-meshheading:10825133-Retinoblastoma Protein, pubmed-meshheading:10825133-Tumor Suppressor Protein p53, pubmed-meshheading:10825133-Up-Regulation
pubmed:year
2000
pubmed:articleTitle
High and low fluences of alpha-particles induce a G1 checkpoint in human diploid fibroblasts.
pubmed:affiliation
Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.