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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2000-6-13
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pubmed:abstractText |
Tumor cells may display a multidrug resistant phenotype by overexpression of ATP-binding cassette transporters such as multidrug resistance (MDRI) P-glycoprotein, multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP). The presence of BCRP has thus far been reported solely using mRNA data. In this study, we describe a BCRP-specific monoclonal antibody, BXP-34, obtained from mice, immunized with mitoxantrone-resistant, BCRP mRNA-positive MCF-7 MR human breast cancer cells. BCRP was detected in BCRP-transfected cells and in several mitoxantrone- and topotecan-selected tumor cell sublines. Pronounced staining of the cell membranes showed that the transporter is mainly present at the plasma membrane. In a panel of human tumors, including primary tumors as well as drug-treated breast cancer and acute myeloid leukemia samples, BCRP was low or undetectable. Extended studies will be required to analyze the possible contribution of BCRP to clinical multidrug resistance.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:AllenJ DJD,
pubmed-author:DaltonW SWS,
pubmed-author:MaliepaardMM,
pubmed-author:PijnenborgA CAC,
pubmed-author:RossD DDD,
pubmed-author:SchefferG LGL,
pubmed-author:SchellensJ HJH,
pubmed-author:ScheperR JRJ,
pubmed-author:SchroeijersA BAB,
pubmed-author:de JongM CMC,
pubmed-author:van GastelenM AMA,
pubmed-author:van der KolkD MDM,
pubmed-author:van der ValkPP
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2589-93
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10825126-ATP-Binding Cassette Transporters,
pubmed-meshheading:10825126-Animals,
pubmed-meshheading:10825126-Antibodies, Monoclonal,
pubmed-meshheading:10825126-Antineoplastic Agents,
pubmed-meshheading:10825126-Breast Neoplasms,
pubmed-meshheading:10825126-Cell Membrane,
pubmed-meshheading:10825126-Drug Resistance, Multiple,
pubmed-meshheading:10825126-Drug Resistance, Neoplasm,
pubmed-meshheading:10825126-Female,
pubmed-meshheading:10825126-Humans,
pubmed-meshheading:10825126-Mice,
pubmed-meshheading:10825126-Mice, Inbred BALB C,
pubmed-meshheading:10825126-Mitoxantrone,
pubmed-meshheading:10825126-Neoplasm Proteins,
pubmed-meshheading:10825126-Topotecan,
pubmed-meshheading:10825126-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Breast cancer resistance protein is localized at the plasma membrane in mitoxantrone- and topotecan-resistant cell lines.
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pubmed:affiliation |
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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