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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-9
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pubmed:abstractText |
Liposomes composed of egg phosphatidylcholine (EPC) (13.4%, of the acyl chains being polyunsaturated fatty acids (PUFA)) and EPC/cholesterol (10:1 mol/mol) were studied for factors that affect liposomal lipid oxidative damage and hydrolysis upon long-term (16 months) storage. Factors studied include: (1) levels of lipid/water interface hydration, related to the presence of cholesterol in the lipid bilayer; (2) the membrane-associated antioxidant vitamin E; (3) the water-soluble antioxidant Tempol; and (4) exposure to light. Liposomal dispersions were stored at room temperature, either exposed to or protected from daylight, for a period of 16 months. Chemical and physical changes were monitored at several time points to assess oxidative and hydrolytic degradation of liposomal lipids. The conclusions of the study are: (1) PUFA are the most sensitive component of the liposome bilayer to oxidative degradation damage during long-term storage; (2) EPC liposomes are more sensitive to degradation during storage than EPC cholesterol liposomes, the presence of cholesterol in the lipid bilayer having a protective effect, probably due to its effect in decreasing the lipid-bilayer hydration; (3) oxidative degradation is the major process during long-term storage, having an earlier onset than the hydrolytic degradation: and (4) Tempol provided significantly better protection than vitamin E to EPC liposomal PUFA against oxidative damage during long-term storage. The relevance of cholesterol's presence, as a 'drying agent', in membranes containing PUFA to resistance of biological membranes to oxidative damage is discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Naphthylamine,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic N-Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Laurates,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Radiation-Protective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Spin Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin E,
http://linkedlifedata.com/resource/pubmed/chemical/laurdan,
http://linkedlifedata.com/resource/pubmed/chemical/tempol
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0009-3084
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10823461-2-Naphthylamine,
pubmed-meshheading:10823461-Antioxidants,
pubmed-meshheading:10823461-Cholesterol,
pubmed-meshheading:10823461-Chromatography, Gas,
pubmed-meshheading:10823461-Chromatography, High Pressure Liquid,
pubmed-meshheading:10823461-Cyclic N-Oxides,
pubmed-meshheading:10823461-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:10823461-Fatty Acids, Unsaturated,
pubmed-meshheading:10823461-Fluorescent Dyes,
pubmed-meshheading:10823461-Hydrogen-Ion Concentration,
pubmed-meshheading:10823461-Laurates,
pubmed-meshheading:10823461-Light,
pubmed-meshheading:10823461-Lipid Bilayers,
pubmed-meshheading:10823461-Lipid Metabolism,
pubmed-meshheading:10823461-Lipids,
pubmed-meshheading:10823461-Liposomes,
pubmed-meshheading:10823461-Membrane Lipids,
pubmed-meshheading:10823461-Ovum,
pubmed-meshheading:10823461-Phosphatidylcholines,
pubmed-meshheading:10823461-Radiation-Protective Agents,
pubmed-meshheading:10823461-Spin Labels,
pubmed-meshheading:10823461-Time Factors,
pubmed-meshheading:10823461-Vitamin E
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pubmed:year |
2000
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pubmed:articleTitle |
Damage to liposomal lipids: protection by antioxidants and cholesterol-mediated dehydration.
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pubmed:affiliation |
Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel. samunia@cc.huji.ac.il
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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