Linked Life Data

10821688

Source:http://linkedlifedata.com/resource/pubmed/id/10821688

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2000-6-21
pubmed:abstractText
Hsp27 kinase activities were studied in adult rat ventricular myocytes following sequential chromatography on Mono Q and Mono S. A basal level of activity was present following cell isolation. FPLC on Mono Q revealed three peaks of activity, peaks 'a', 'b', and 'c'. A fourth peak, 'd', was detected upon subsequent chromatography of the Mono Q flow-through on Mono S. Immunoblotting revealed that peaks 'a', 'b', and 'c' contained predominantly a 49 kDa form of MAPKAP kinase-2. Peak 'd' contained a 43 kDa form. 'In-gel' kinase assays using hsp27 indicated both forms of MAPKAP kinase-2 were active. No other bands of hsp27 kinase activity were detected. Both forms of hsp27 kinase immunoprecipitated with a MAPKAP kinase-2 antibody and have therefore been named MAPKAP kinase-2alpha (p49) and MAPKAP kinase-2beta (p43). MAPKAP kinase-2beta chromatographed on Superose 12 as a 60.7 kDa monomer whereas the behavior of MAPKAP kinase-2alpha suggested both a 65.7 kDa monomer and higher molecular mass complexes. Both activities phosphorylated hsp27 on serine residues, and two-dimensional phosphopeptide mapping indicated the same sites were phosphorylated. A tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA), stimulated both MAPKAP kinase-2alpha and MAPKAP kinase-2beta activity. Inhibition of MEK activation with PD 98059 or p38alpha/beta MAP kinase activity with SB203580 blocked activation by PMA. However, whereas PD 98059 inhibited only the PMA-stimulated activation, SB203580 inhibited both PMA-stimulated and basal hsp27 phosphorylation. These data demonstrate the presence of two forms of MAPKAP kinase-2 in adult ventricular myocytes. Both forms are activated indirectly by the ERK MAP kinase pathway and directly by p38 MAP kinase but independently regulated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6145-56
pubmed:dateRevised
2010-10-4
pubmed:meshHeading
pubmed-meshheading:10821688-Amino Acid Sequence, pubmed-meshheading:10821688-Animals, pubmed-meshheading:10821688-Chromatography, Ion Exchange, pubmed-meshheading:10821688-Dogs, pubmed-meshheading:10821688-HSP27 Heat-Shock Proteins, pubmed-meshheading:10821688-HeLa Cells, pubmed-meshheading:10821688-Heat-Shock Proteins, pubmed-meshheading:10821688-Humans, pubmed-meshheading:10821688-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:10821688-Isoenzymes, pubmed-meshheading:10821688-Male, pubmed-meshheading:10821688-Molecular Sequence Data, pubmed-meshheading:10821688-Myocardium, pubmed-meshheading:10821688-Neoplasm Proteins, pubmed-meshheading:10821688-PC12 Cells, pubmed-meshheading:10821688-Phosphorylation, pubmed-meshheading:10821688-Precipitin Tests, pubmed-meshheading:10821688-Protein-Serine-Threonine Kinases, pubmed-meshheading:10821688-Rabbits, pubmed-meshheading:10821688-Rats, pubmed-meshheading:10821688-Rats, Sprague-Dawley
pubmed:year
2000
pubmed:articleTitle
Two distinct forms of MAPKAP kinase-2 in adult cardiac ventricular myocytes.
pubmed:affiliation
Institut de Cardiologie de Montréal, Centre de Recherche, 5000 rue Bélanger est, Montréal, PQ, Canada, H1T 1C8.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't