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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2000-6-21
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244928,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244929,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244930,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244931,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244932,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244933,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244934,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244935,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244936,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244937,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244938,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244939,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244940,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244941,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244942,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244943,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244944,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244945,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244946,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244947,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244948,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244949,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244950,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244951,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244952,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244953,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244954,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244955,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244956,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AJ244957
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pubmed:abstractText |
The VH4 genes expressed by both resting and in vivo-activated subepithelial (SE) B cells from human tonsils were studied. Resting SE B cells were subdivided according to the presence (IgDlow) or absence (IgM-only) of surface IgD. CD27 was abundant on activated SE B cells and low on resting IgM-only B cells. Resting IgDlow SE B cells could be subdivided into CD27low and CD27high cell fractions. Resting IgDlow SE B cells displayed VH4 genes with a substantial number of mutations (13/29 of the molecular clones were mutated), whereas 25/26 of the clones from resting IgM-only SE B cells were unmutated. Moreover, mutated VH4 genes were detected mainly within the CD27high cell fraction of the IgDlow SE B cells. Several identical unmutated VH4DJH sequences (11/32) were found in different molecular clones from resting IgM-only SE B cells, suggesting local cellular expansion. Both unmutated (14/25) and mutated (11/25) sequences were found in mu transcripts of activated SE B cells. Extensive mutation was observed in the gamma transcripts of activated SE B cells. Therefore, SE B cells are heterogeneous, being comprised of B cells with mutated Ig VH4 genes, that are Ag-experienced B cells, and a subset of B cells with unmutated VH4 genes that are either virgin cells or cells driven by Ags that did not induce or select for V gene mutations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5596-604
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10820234-Antigens, CD27,
pubmed-meshheading:10820234-B-Lymphocyte Subsets,
pubmed-meshheading:10820234-Child,
pubmed-meshheading:10820234-Child, Preschool,
pubmed-meshheading:10820234-Epithelium,
pubmed-meshheading:10820234-Gene Expression Regulation,
pubmed-meshheading:10820234-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:10820234-Germ-Line Mutation,
pubmed-meshheading:10820234-Germinal Center,
pubmed-meshheading:10820234-Humans,
pubmed-meshheading:10820234-Immunoglobulin D,
pubmed-meshheading:10820234-Immunoglobulin M,
pubmed-meshheading:10820234-Immunoglobulin Variable Region,
pubmed-meshheading:10820234-Immunophenotyping,
pubmed-meshheading:10820234-Interphase,
pubmed-meshheading:10820234-Lymphocyte Activation,
pubmed-meshheading:10820234-Molecular Sequence Data,
pubmed-meshheading:10820234-Palatine Tonsil,
pubmed-meshheading:10820234-Spleen
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pubmed:year |
2000
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pubmed:articleTitle |
Heterogeneity of tonsillar subepithelial B lymphocytes, the splenic marginal zone equivalents.
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pubmed:affiliation |
Servizio di Immunologia Clinica and Servizio di Citometria Centro Biotechnologie Avanzate, Istituto Nazionale per la Ricerca sul Cancro, IST, Genova, Italy. mdono@hp380.ist.unige.it
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pubmed:publicationType |
Journal Article,
Comparative Study
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