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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
31
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pubmed:dateCreated |
2000-9-7
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pubmed:abstractText |
We previously identified Rho-associated protein kinase (Rho-kinase) as a specific effector of Rho. In this study, we identified collapsin response mediator protein-2 (CRMP-2), as a novel Rho-kinase substrate in the brain. CRMP-2 is a neuronal protein whose expression is up-regulated during development. Rho-kinase phosphorylated CRMP-2 at Thr-555 in vitro. We produced an antibody that specifically recognizes CRMP-2 phosphorylated at Thr-555. Using this antibody, we found that Rho-kinase phosphorylated CRMP-2 downstream of Rho in COS7 cells. Phosphorylation of CRMP-2 was observed in chick dorsal root ganglion neurons during lysophosphatidic acid (LPA)-induced growth cone collapse, whereas the phosphorylation was not detected during semaphorin-3A-induced growth cone collapse. Both LPA-induced CRMP-2 phosphorylation and LPA-induced growth cone collapse were inhibited by Rho-kinase inhibitor HA1077 or Y-32885. LPA-induced growth cone collapse was also blocked by a dominant negative form of Rho-kinase. On the other hand, semaphorin-3A-induced growth cone collapse was not inhibited by a dominant negative form of Rho-kinase. Furthermore, overexpression of a mutant CRMP-2 in which Thr-555 was replaced by Ala significantly inhibited LPA-induced growth cone collapse. These results demonstrate the existence of Rho-kinase-dependent and -independent pathways for growth cone collapse and suggest that CRMP-2 phosphorylation by Rho-kinase is involved in the former pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Semaphorin-3A,
http://linkedlifedata.com/resource/pubmed/chemical/collapsin response mediator...,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23973-80
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10818093-Amino Acid Sequence,
pubmed-meshheading:10818093-Animals,
pubmed-meshheading:10818093-Antibodies,
pubmed-meshheading:10818093-Brain Chemistry,
pubmed-meshheading:10818093-COS Cells,
pubmed-meshheading:10818093-Cattle,
pubmed-meshheading:10818093-Ganglia, Spinal,
pubmed-meshheading:10818093-Humans,
pubmed-meshheading:10818093-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10818093-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10818093-Lysophospholipids,
pubmed-meshheading:10818093-Molecular Sequence Data,
pubmed-meshheading:10818093-Nerve Tissue Proteins,
pubmed-meshheading:10818093-Neurons,
pubmed-meshheading:10818093-Phosphoproteins,
pubmed-meshheading:10818093-Phosphorylation,
pubmed-meshheading:10818093-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10818093-Recombinant Proteins,
pubmed-meshheading:10818093-Semaphorin-3A,
pubmed-meshheading:10818093-Substrate Specificity,
pubmed-meshheading:10818093-rho-Associated Kinases
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pubmed:year |
2000
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pubmed:articleTitle |
Phosphorylation of collapsin response mediator protein-2 by Rho-kinase. Evidence for two separate signaling pathways for growth cone collapse.
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pubmed:affiliation |
Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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