Source:http://linkedlifedata.com/resource/pubmed/id/10816659
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2000-6-2
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pubmed:abstractText |
We evidenced in vitro proopiomelanocortin (POMC) mRNA-transcription in human dermal fibroblasts using Northern blot hybridization. Modulation of POMC gene expression by cytokines (transforming growth factor-beta, TGF-beta, and tumor necrosis factor-alpha, TNF-alpha) was investigated by incubating human normal fibroblasts with 1 and 10 ng/ml cytokines, either alone or in combination, for 24 hours. Our results show that dermal fibroblasts express POMC at significant levels under unstimulated conditions. POMC steady-state levels were significantly reduced by addition of TGF-beta. On the other hand, TNF-alpha exerted a stimulatory effect on POMC mRNA transcription, partially counteracting the effect of TGF-beta. These data provide the first demonstration of POMC gene expression in cultured skin fibroblasts. The opposite regulatory effect of TGF-beta and TNF-alpha, two cytokines primarily involved in extracellular matrix regulation, suggests a possible role for POMC-derived peptides in fibroblast activity. We also investigated POMC mRNA expression in keloid-derived fibroblasts in culture, and its regulation by TGF-beta added at the highest concentration documented for inhibition. Keloid-derived fibroblasts showed clearly detectable levels of POMC mRNA in basal conditions, and no alteration of POMC gene expression was observed when TGF-beta was added in culture. The altered TGF-beta regulation of POMC mRNA levels suggest that POMC-derived peptides may play a role in the pathogenesis of keloid formation through an autocrine/paracrine network, resulting in modulation of extracellular matrix synthesis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Pro-Opiomelanocortin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0077-8923
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
885
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
262-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10816659-Cells, Cultured,
pubmed-meshheading:10816659-Fibroblasts,
pubmed-meshheading:10816659-Gene Expression Regulation,
pubmed-meshheading:10816659-Humans,
pubmed-meshheading:10816659-Keloid,
pubmed-meshheading:10816659-Keratinocytes,
pubmed-meshheading:10816659-Kinetics,
pubmed-meshheading:10816659-Pro-Opiomelanocortin,
pubmed-meshheading:10816659-Recombinant Proteins,
pubmed-meshheading:10816659-Skin Physiological Phenomena,
pubmed-meshheading:10816659-Transforming Growth Factor beta,
pubmed-meshheading:10816659-Tumor Necrosis Factor-alpha
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pubmed:year |
1999
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pubmed:articleTitle |
POMC and fibroblast biology.
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pubmed:affiliation |
Department of Dermatology, University of Florence, Italy.
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pubmed:publicationType |
Journal Article
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