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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2000-6-22
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pubmed:databankReference |
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pubmed:abstractText |
Pax5 (BSAP) functions as both a transcriptional activator and repressor during midbrain patterning, B-cell development and lymphomagenesis. Here we demonstrate that Pax5 exerts its repression function by recruiting members of the Groucho corepressor family. In a yeast two-hybrid screen, the groucho-related gene product Grg4 was identified as a Pax5 partner protein. Both proteins interact cooperatively via two separate domains: the N-terminal Q and central SP regions of Grg4, and the octapeptide motif and C-terminal transactivation domain of Pax5. The phosphorylation state of Grg4 is altered in vivo upon Pax5 binding. Moreover, Grg4 efficiently represses the transcriptional activity of Pax5 in an octapeptide-dependent manner. Similar protein interactions resulting in transcriptional repression were also observed between distantly related members of both the Pax2/5/8 and Groucho protein families. In agreement with this evolutionary conservation, the octapeptide motif of Pax proteins functions as a Groucho-dependent repression domain in Drosophila embryos. These data indicate that Pax proteins can be converted from transcriptional activators to repressors through interaction with corepressors of the Groucho protein family.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10811620-10022895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10811620-10197586,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10811620-9887105
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2292-303
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10811620-3T3 Cells,
pubmed-meshheading:10811620-Animals,
pubmed-meshheading:10811620-B-Cell-Specific Activator Protein,
pubmed-meshheading:10811620-DNA-Binding Proteins,
pubmed-meshheading:10811620-Mice,
pubmed-meshheading:10811620-Molecular Sequence Data,
pubmed-meshheading:10811620-Proteins,
pubmed-meshheading:10811620-Repressor Proteins,
pubmed-meshheading:10811620-Trans-Activators,
pubmed-meshheading:10811620-Transcription, Genetic,
pubmed-meshheading:10811620-Transcription Factors,
pubmed-meshheading:10811620-Transcriptional Activation
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pubmed:year |
2000
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pubmed:articleTitle |
Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family.
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pubmed:affiliation |
Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, A-1030 Vienna, Austria and Departamento de Biologia Molecular i Cellular, CID-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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