Linked Life Data

10806336

Source:http://linkedlifedata.com/resource/pubmed/id/10806336

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pubmed-article:10806336pubmed:abstractTextThe effect of changing cell volume on rat mammary protein synthesis has been examined. Cell swelling, induced by a hyposmotic challenge, markedly increased the incorporation of radiolabelled amino acids (leucine and methionine) into trichloroacetic acid (TCA)-precipitable material: reducing the osmolality by 47% increased leucine and methionine incorporation into mammary protein by 147 and 126% respectively. Conversely, cell shrinking, induced by a hyperosmotic shock, almost abolished the incorporation of radiolabelled amino acids into mammary protein: increasing the osmolality by 70% reduced leucine and methionine incorporation into mammary protein by 86 and 93% respectively. The effects of cell swelling and shrinking were fully reversible. Volume-sensitive mammary tissue protein synthesis was dependent upon the extent of the osmotic challenge. Isosmotic swelling of mammary tissue, using a buffer containing urea (160 mM), increased the incorporation of radiolabelled leucine into TCA-precipitable material by 106%. Swelling-induced mammary protein synthesis was dependent upon calcium: removing extracellular calcium together with the addition of EGTA markedly reduced volume-activated protein synthesis. Cell swelling-induced protein synthesis was inhibited by the Ca(2+) ATPase blocker thapsigargin suggesting that volume-sensitive protein synthesis is dependent upon luminal calcium.lld:pubmed
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pubmed-article:10806336pubmed:authorpubmed-author:ZammitV AVAlld:pubmed
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pubmed-article:10806336pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10806336pubmed:articleTitleRegulation of protein synthesis in lactating rat mammary tissue by cell volume.lld:pubmed
pubmed-article:10806336pubmed:affiliationHannah Research Institute, Ayr, Scotland, UK.lld:pubmed
pubmed-article:10806336pubmed:publicationTypeJournal Articlelld:pubmed
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pubmed-article:10806336pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed