Linked Life Data

10806228

Source:http://linkedlifedata.com/resource/pubmed/id/10806228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-23
pubmed:abstractText
Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by BR. Through this screening experiment, the compound brassinazole was selected as the most potent chemical. In dark-grown Arabidopsis, brassinazole-induced morphological changes were nearly restored to those of wild type by treatment with brassinolide. The structure of brassinazole is similar to pacrobutrazol, a gibberellin biosynthesis inhibitor. However, in assays with cress (Lepidium sativum) plants, brassinazole-treated plants did not show recovery after the addition of gibberellin but showed good recovery after the addition of brassinolide. These data demonstrate that brassinazole is a specific BR biosynthesis inhibitor. Brassinazole-treated cress also showed dwarfism, with altered leaf morphology, including the downward curling and dark green color typical of Arabidopsis BR-deficient mutants, and this dwarfism was reversed by the application of 10 nM brassinolide. This result suggests that BRs are essential for plant growth, and that brassinazole can be used to clarify the function of BRs in plants as a complement to BR-deficient mutants. The brassinazole action site was also investigated by feeding BR biosynthesis intermediates to cress grown in the light.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-10069828, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-10091696, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-2065972, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-8055176, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-8400871, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-8602526, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-8612270, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-8919915, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9178548, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9401120, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9440936, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9490745, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9490746, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9927639, http://linkedlifedata.com/resource/pubmed/commentcorrection/10806228-9990098
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-100
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor.
pubmed:affiliation
The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan. tasami@postman.riken.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't