| pubmed-article:10805458 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10805458 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:10805458 | lifeskim:mentions | umls-concept:C0037081 | lld:lifeskim |
| pubmed-article:10805458 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10805458 | pubmed:dateCreated | 2000-7-28 | lld:pubmed |
| pubmed-article:10805458 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:abstractText | A previously unidentified extension of an open reading frame from the genomic DNA of Japonica rice (Oryza sativa L.) encoding oryzacystatin-I (OC-I; access. M29259, protein ID AAA33912.1) has been identified as a 5' gene segment coding for the OC-I signal peptide. The signal peptide appears to direct a pre-protein (SPOC-I; Accession No. AF164378) to the endoplasmic reticulum, where it is processed into the mature form of OC-I. The start codon of SPOC-I begins 114 bp upstream from that previously published for OC-I. A putative proteolytic site. which may yield a mature OC-I approximately 12 residues larger than previously described, has been identified within SPOC-I between Ala-26 and Glu-27. The signal peptide sequence was amplified by polymerase chain reaction using genomic DNA from O. sativa seedlings and ligated to the 5' end of the truncated OC-I gene at the endogenous SalI site. Partially purified protein extracts from Escherichia coli expressing SPOC-I reacted with polyclonal antibodies raised against OC-I and revealed a protein of the expected molecular weight (15,355 Da). In-vitro translation of SPOC-I in the presence of microsomal membranes yielded a processed product approximately 2.7 kDa smaller than the pre-protein. Nicotiana tabacum L. cv. Xanthi plants independently transformed with the SPOC-I gene processed SPOC-I and accumulated the mature form of OC-I (approximately 12.6 kDa), which co-migrated with natural, mature OC-I extracted from rice seed when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | lld:pubmed |
| pubmed-article:10805458 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10805458 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10805458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10805458 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10805458 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10805458 | pubmed:issn | 0032-0935 | lld:pubmed |
| pubmed-article:10805458 | pubmed:author | pubmed-author:KempJ DJD | lld:pubmed |
| pubmed-article:10805458 | pubmed:author | pubmed-author:RandallJJ | lld:pubmed |
| pubmed-article:10805458 | pubmed:author | pubmed-author:WomackJ SJS | lld:pubmed |
| pubmed-article:10805458 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10805458 | pubmed:volume | 210 | lld:pubmed |
| pubmed-article:10805458 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10805458 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10805458 | pubmed:pagination | 844-7 | lld:pubmed |
| pubmed-article:10805458 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10805458 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10805458 | pubmed:articleTitle | Identification of a signal peptide for oryzacystatin-I. | lld:pubmed |
| pubmed-article:10805458 | pubmed:affiliation | Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces 88003, USA. | lld:pubmed |
| pubmed-article:10805458 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10805458 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:10805458 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10805458 | lld:pubmed |
