Source:http://linkedlifedata.com/resource/pubmed/id/10805458
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2000-7-28
|
| pubmed:databankReference | |
| pubmed:abstractText |
A previously unidentified extension of an open reading frame from the genomic DNA of Japonica rice (Oryza sativa L.) encoding oryzacystatin-I (OC-I; access. M29259, protein ID AAA33912.1) has been identified as a 5' gene segment coding for the OC-I signal peptide. The signal peptide appears to direct a pre-protein (SPOC-I; Accession No. AF164378) to the endoplasmic reticulum, where it is processed into the mature form of OC-I. The start codon of SPOC-I begins 114 bp upstream from that previously published for OC-I. A putative proteolytic site. which may yield a mature OC-I approximately 12 residues larger than previously described, has been identified within SPOC-I between Ala-26 and Glu-27. The signal peptide sequence was amplified by polymerase chain reaction using genomic DNA from O. sativa seedlings and ligated to the 5' end of the truncated OC-I gene at the endogenous SalI site. Partially purified protein extracts from Escherichia coli expressing SPOC-I reacted with polyclonal antibodies raised against OC-I and revealed a protein of the expected molecular weight (15,355 Da). In-vitro translation of SPOC-I in the presence of microsomal membranes yielded a processed product approximately 2.7 kDa smaller than the pre-protein. Nicotiana tabacum L. cv. Xanthi plants independently transformed with the SPOC-I gene processed SPOC-I and accumulated the mature form of OC-I (approximately 12.6 kDa), which co-migrated with natural, mature OC-I extracted from rice seed when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cystatins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0032-0935
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
210
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
844-7
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10805458-Amino Acid Sequence,
pubmed-meshheading:10805458-Base Sequence,
pubmed-meshheading:10805458-Blotting, Western,
pubmed-meshheading:10805458-Cystatins,
pubmed-meshheading:10805458-DNA, Plant,
pubmed-meshheading:10805458-DNA, Recombinant,
pubmed-meshheading:10805458-Microsomes,
pubmed-meshheading:10805458-Molecular Sequence Data,
pubmed-meshheading:10805458-Oryza sativa,
pubmed-meshheading:10805458-Plants, Genetically Modified,
pubmed-meshheading:10805458-Plants, Toxic,
pubmed-meshheading:10805458-Plasmids,
pubmed-meshheading:10805458-Protein Biosynthesis,
pubmed-meshheading:10805458-Protein Precursors,
pubmed-meshheading:10805458-Protein Processing, Post-Translational,
pubmed-meshheading:10805458-Protein Sorting Signals,
pubmed-meshheading:10805458-Sequence Analysis, DNA,
pubmed-meshheading:10805458-Tobacco,
pubmed-meshheading:10805458-Transformation, Genetic
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| pubmed:year |
2000
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| pubmed:articleTitle |
Identification of a signal peptide for oryzacystatin-I.
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| pubmed:affiliation |
Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces 88003, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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