Linked Life Data

10802062

Source:http://linkedlifedata.com/resource/pubmed/id/10802062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-6-5
pubmed:abstractText
Gelsolin is an actin filament severing protein composed of six similar structured domains that differ with respect to actin, calcium and polyphospho-inositide binding. Previous work has established that gelsolin binds tropomyosin [Koepf, E.K. and Burtnick, L.D. (1992) FEBS Lett. 309, 56-58]. We have produced various specific gelsolin domains in Escherichia coli in order to establish which of the six domains binds tropomyosin. Gelsolin domains 1-3 (G1-3), G1-2 and G2 all bind tropomyosin in a pH and calcium insensitive manner whereas binding of G4-6 to tropomyosin was barely detectable under the conditions tested. We conclude that gelsolin binds tropomyosin via domain 2 (G2).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
473
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10802062-Animals, pubmed-meshheading:10802062-Binding, Competitive, pubmed-meshheading:10802062-Calcium, pubmed-meshheading:10802062-Chemical Precipitation, pubmed-meshheading:10802062-Chromatography, Affinity, pubmed-meshheading:10802062-Gelsolin, pubmed-meshheading:10802062-Humans, pubmed-meshheading:10802062-Hydrogen-Ion Concentration, pubmed-meshheading:10802062-Light, pubmed-meshheading:10802062-Magnesium, pubmed-meshheading:10802062-Muscle, Skeletal, pubmed-meshheading:10802062-Peptide Fragments, pubmed-meshheading:10802062-Protein Binding, pubmed-meshheading:10802062-Protein Structure, Tertiary, pubmed-meshheading:10802062-Rabbits, pubmed-meshheading:10802062-Recombinant Proteins, pubmed-meshheading:10802062-Scattering, Radiation, pubmed-meshheading:10802062-Tropomyosin, pubmed-meshheading:10802062-Turkeys, pubmed-meshheading:10802062-Ultracentrifugation
pubmed:year
2000
pubmed:articleTitle
Domain 2 of gelsolin binds directly to tropomyosin.
pubmed:affiliation
Genes and Development Group, Department of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, UK. skm@srv4.med.ed.ac.uk
pubmed:publicationType
Journal Article