Linked Life Data

10801832

Source:http://linkedlifedata.com/resource/pubmed/id/10801832

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2000-8-16
pubmed:abstractText
The mechanisms that control the emergence of different anterior pituitary cells from a common stem cell population are largely unknown. The immortalized GHFT cells derived from targeted expression of SV40 T antigen to mouse pituitary display characteristics of somatolactotropic progenitors in that they express the transcription factor GHF-1 (Pit-1) but not growth hormone (GH) or prolactin (PRL). We searched for factors capable of inducing lactotropic differentiation of GHFT cells. PRL gene expression was not observed in cells subjected to a variety of stimuli, which induce PRL gene expression in mature lactotropes. Only fibroblast growth factor-2 (FGF-2) was able to initiate the transcription, synthesis, and release of PRL in GHFT cells. However, induction of PRL expression was incomplete in FGF-2-treated cells, suggesting that additional factors are necessary to attain high levels of PRL transcription in fully differentiated lactotropes. We also show that the FGF-2 response element is located in the proximal PRL promoter. Stimulation of PRL expression by FGF-2 requires endogenous Ets factors and these factors as well as GHF-1 are expressed at low levels in the committed precursor, suggesting that these low levels are limiting for full PRL expression. Moreover, FGF-2 effect on lactotrope differentiation is mediated, at least partially, by stimulation of the Ras-signaling pathway. Our results suggest that, indeed, GHFT cells represent a valid model for studying lactotropic differentiation and that FGF-2 could play a key role both in initiating lactotrope differentiation and maintaining PRL expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21653-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10801832-Animals, pubmed-meshheading:10801832-Antigens, Polyomavirus Transforming, pubmed-meshheading:10801832-Cell Differentiation, pubmed-meshheading:10801832-Cell Line, Transformed, pubmed-meshheading:10801832-DNA-Binding Proteins, pubmed-meshheading:10801832-Gene Expression Regulation, pubmed-meshheading:10801832-Kinetics, pubmed-meshheading:10801832-Mice, pubmed-meshheading:10801832-Pituitary Gland, Anterior, pubmed-meshheading:10801832-Prolactin, pubmed-meshheading:10801832-Proto-Oncogene Proteins, pubmed-meshheading:10801832-Proto-Oncogene Proteins c-ets, pubmed-meshheading:10801832-RNA, Messenger, pubmed-meshheading:10801832-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10801832-Simian virus 40, pubmed-meshheading:10801832-Stem Cells, pubmed-meshheading:10801832-Transcription, Genetic, pubmed-meshheading:10801832-Transcription Factor Pit-1, pubmed-meshheading:10801832-Transcription Factors
pubmed:year
2000
pubmed:articleTitle
Differentiation of lactotrope precursor GHFT cells in response to fibroblast growth factor-2.
pubmed:affiliation
Department of Pharmacology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0636, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't