Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2000-6-7
pubmed:abstractText
B cells of the largest Ig variable heavy chain gene (VH) family, VH3, are reportedly decreased in patients with late stage HIV-1 disease. This deficit may contribute to their impaired responses to infections and vaccines. We confirmed that the VH3 family was underrepresented in serum IgM proteins, with a 45% decrease in patients with advanced HIV-1 disease. However, the proportion of VH3 within VH(1-6) IgM mRNA from peripheral B cells did not differ from that of control subjects (mean +/- SD, 57.1 +/- 9.7 vs 61.1 +/- 8. 7%). Similarly, within VH(1-6) IgD mRNA, which even more closely represents the unstimulated naive repertoire, the relative expression of VH3 mRNA was comparable in the two groups. Moreover, the frequency of individual genes within the VH3 family for IgD, particularly genes which encode putative HIV-1 gp120 binding sites, also was normal in HIV-1-infected patients. However, VH3 family expression for IgG mRNA was significantly decreased (17%) and VH4 IgG was increased (33%) relative to other VH families in advanced HIV-1-infected patients. Thus, the changes in VH family expression were more readily apparent in previously activated IgG "memory" B cell populations and, likely, in cells actively producing IgM rather than in resting naive cells. The presence of a relatively normal naive VH3 IgM and IgD mRNA repertoire in resting cells supports the prospect that with proper stimulation, particularly in conjunction with effective antiviral therapy, vigorous humoral immune responses to infections and vaccines may be elicited in this high-risk population.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5482-91
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10799916-B-Lymphocyte Subsets, pubmed-meshheading:10799916-Cell Differentiation, pubmed-meshheading:10799916-Cell Line, pubmed-meshheading:10799916-Gene Expression Regulation, pubmed-meshheading:10799916-Gene Frequency, pubmed-meshheading:10799916-Genes, Immunoglobulin, pubmed-meshheading:10799916-HIV Infections, pubmed-meshheading:10799916-HIV-1, pubmed-meshheading:10799916-Humans, pubmed-meshheading:10799916-Immunoglobulin D, pubmed-meshheading:10799916-Immunoglobulin G, pubmed-meshheading:10799916-Immunoglobulin Heavy Chains, pubmed-meshheading:10799916-Immunoglobulin M, pubmed-meshheading:10799916-Immunoglobulin Variable Region, pubmed-meshheading:10799916-Immunologic Memory, pubmed-meshheading:10799916-Interphase, pubmed-meshheading:10799916-Multigene Family, pubmed-meshheading:10799916-RNA, Messenger
pubmed:year
2000
pubmed:articleTitle
Impact of HIV-1 infection on VH3 gene repertoire of naive human B cells.
pubmed:affiliation
Center for Mucosal and Vaccine Biology, Infectious Disease Section, Veteran Affairs Medical Center, University of Minnesota School of Medicine, Minneapolis, MN 55417, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.