Source:http://linkedlifedata.com/resource/pubmed/id/10799547
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
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pubmed:dateCreated |
2000-6-8
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pubmed:abstractText |
Many membrane-bound proteins, including cytokines, receptors, and growth factors, are proteolytically cleaved to release a soluble form of their extracellular domain. The tumor necrosis factor (TNF)-alpha converting enzyme (TACE/ADAM-17) is a transmembrane metalloproteinase responsible for the proteolytic release or "shedding" of several cell-surface proteins, including TNF and p75 TNFR. We established a TACE-reconstitution system using TACE-deficient cells co-transfected with TACE and substrate cDNAs to study TACE function and regulation. Using the TACE-reconstitution system, we identified two additional substrates of TACE, interleukin (IL)-1R-II and p55 TNFR. Using truncations and chimeric constructs of TACE and another ADAM family member, ADAM-10, we studied the function of the different domains of TACE in three shedding activities. We found that TACE must be expressed with its membrane-anchoring domain for phorbol ester-stimulated shedding of TNF, p75 TNFR, and IL-1R-II, but that the cytoplasmic domain is not required for the shedding of these substrates. The catalytic domain of ADAM-10 could not be functionally substituted for that of TACE. IL-1R-II shedding required the cysteine-rich domain of TACE as well as the catalytic domain, whereas TNF and p75 TNFR shedding required only the tethered TACE catalytic domain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADAM Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/tumor necrosis factor-alpha...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14608-14
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10799547-ADAM Proteins,
pubmed-meshheading:10799547-Animals,
pubmed-meshheading:10799547-Antigens, CD,
pubmed-meshheading:10799547-Catalytic Domain,
pubmed-meshheading:10799547-Cell Line,
pubmed-meshheading:10799547-Cytoplasm,
pubmed-meshheading:10799547-DNA, Complementary,
pubmed-meshheading:10799547-Metalloendopeptidases,
pubmed-meshheading:10799547-Mice,
pubmed-meshheading:10799547-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:10799547-Receptors, Tumor Necrosis Factor, Type II,
pubmed-meshheading:10799547-Transfection,
pubmed-meshheading:10799547-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme.
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pubmed:affiliation |
Department of Cell Sciences, Immunex Corporation, Seattle, Washington 98101, USA.
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pubmed:publicationType |
Journal Article
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